Abstract Nanozymes have been developed as alternative for enzymes to overcome practical limitations of enzymes in industry and medicine. Infectious diseases are becoming severe threat to public health. Hence, peroxidase nanozyme for combating bacteria have been designed. Core-Shell Au@Co-Fe hybrid nanoparticles (Au@Co-Fe NPs) were synthesized. The structure of Au@Co-Fe NPs was characterized by UV–vis and FT-IR spectroscopic methods. The size, zeta potential and spherical morphology of Au@Co-Fe NPs were determined by DLS, TEM and AFM techniques. Au@Co-Fe NPs has been evaluated as peroxidase mimic nanozyme. The peroxidase mimetic activity of gold nanoparticles, Co (II) and Fe (III) were measured and compared with that obtained for native HRP. The enzymatic measured activity was 50% of native horse radish peroxidase. Additionally, Au@Co-Fe NPs was evaluated as antibacterial agent against four selected standard pathogenic bacteria as Escherichia coli, Pseudomonas aeruginosa (as gram negative) and Staphylococcus aureus, and Bacillus cereus (as gram positive).

中文翻译：

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核壳 Au@Co-Fe 杂化纳米粒子作为过氧化物酶模拟纳米酶用于抗菌应用

摘要 纳米酶已被开发作为酶的替代品，以克服酶在工业和医学中的实际局限性。传染病正在成为对公众健康的严重威胁。因此，已经设计了用于对抗细菌的过氧化物酶纳米酶。合成了核壳 Au@Co-Fe 混合纳米粒子（Au@Co-Fe NPs）。Au@Co-Fe NPs的结构通过UV-vis和FT-IR光谱方法表征。Au@Co-Fe NPs 的尺寸、zeta 电位和球形形态通过 DLS、TEM 和 AFM 技术确定。Au@Co-Fe NPs 已被评估为过氧化物酶模拟纳米酶。测量了金纳米粒子 Co (II) 和 Fe (III) 的过氧化物酶模拟活性，并与天然 HRP 获得的活性进行了比较。酶促测量活性是天然辣根过氧化物酶的 50%。此外，