Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a high level disease threat in Europe and also Asia. Examination of suspect clinical cases for ASF with rapid and sensitive laboratory methods can substantially contribute to the detection and characterization of new outbreaks. In this study two sensitive tests were developed for the detection of the p72 major capsid protein of ASFV both in cell culture with an immunocytochemical (IC) and in tissue samples with an immunohistochemical (IHC) method using a commercially available mouse monoclonal antibody (clone 1BC11). The IC test was able to detect the virus at high virus dilutions in cell culture and the IHC test indicated the presence of ASFV in all formalin-fixed and paraffin-embedded tissue samples collected from two wild boars. The reported IC and IHC methods were found to be useful ancillary laboratory tests for research purposes and for the diagnosis of acute ASF.

自2007年将非洲猪瘟病毒（ASFV）引入欧洲以来，ASFV感染在过去几年中一直在不断蔓延，并已成为欧洲乃至亚洲的高度疾病威胁。用快速，灵敏的实验室方法检查可疑的ASF临床病例，可以极大地有助于发现和表征新的暴发。在这项研究中，开发了两个灵敏的测试，用于使用免疫细胞化学（IC）方法检测细胞培养中的ASFV p72主要衣壳蛋白，以及使用市售的小鼠单克隆抗体（克隆1BC11）通过免疫组织化学（IHC）方法检测组织样品中的ASFV p72主要衣壳蛋白。 ）。IC测试能够在细胞培养物中以高病毒稀释度检测到病毒，IHC测试表明，从两个野猪收集的所有福尔马林固定和石蜡包埋的组织样品中均存在ASFV。发现报告的IC和IHC方法对于研究目的和急性ASF的诊断是有用的辅助实验室测试。