Arsenolipids, especially arsenic-containing hydrocarbons (AsHC), are an emerging class of seafood originating contaminants. Here we toxicologically characterize a recently identified oxo-AsHC 332 metabolite, thioxo-AsHC 348 in cultured human liver (HepG2) cells. Compared to results of previous studies of the parent compound oxo-AsHC 332, thioxo-AsHC 348 substantially affected cell viability in the same concentration range but exerted about 10-fold lower cellular bioavailability. Similar to oxo-AsHC 332, thioxo-AsHC 348 did not substantially induce oxidative stress nor DNA damage. Moreover, in contrast to oxo-AsHC 332 mitochondria seem not to be a primary subcellular toxicity target for thioxo-AsHC. This study indicates that thioxo-AsHC 348 is at least as toxic as its parent compound oxo-AsHC 332 but very likely acts via a different mode of toxic action, which still needs to be identified.

砷脂，尤其是含砷的碳氢化合物（AsHC），是一类新兴的源自海鲜的污染物。在这里，我们在培养的人肝（HepG2）细胞中对最近鉴定出的oxo-AsHC 332代谢产物thioxo-AsHC 348进行毒理学表征。与先前对母体化合物oxo-AsHC 332的研究结果相比，thioxo-AsHC 348在相同的浓度范围内会显着影响细胞活力，但其细胞生物利用度却降低了约10倍。类似于oxo-AsHC 332，thioxo-AsHC 348基本上不引起氧化应激或DNA损伤。此外，与oxo-AsHC 332相比，线粒体似乎不是thioxo-AsHC的主要亚细胞毒性靶标。这项研究表明，thioxo-AsHC 348的毒性至少与其母体化合物oxo-AsHC 332一样，但很可能通过 仍然需要确定另一种毒性作用方式。