Arsenolipids, especially arsenic-containing hydrocarbons (AsHC), are an emerging class of seafood originating contaminants. Here we toxicologically characterize a recently identified oxo-AsHC 332 metabolite, thioxo-AsHC 348 in cultured human liver (HepG2) cells. Compared to results of previous studies of the parent compound oxo-AsHC 332, thioxo-AsHC 348 substantially affected cell viability in the same concentration range but exerted about 10-fold lower cellular bioavailability. Similar to oxo-AsHC 332, thioxo-AsHC 348 did not substantially induce oxidative stress nor DNA damage. Moreover, in contrast to oxo-AsHC 332 mitochondria seem not to be a primary subcellular toxicity target for thioxo-AsHC. This study indicates that thioxo-AsHC 348 is at least as toxic as its parent compound oxo-AsHC 332 but very likely acts via a different mode of toxic action, which still needs to be identified.

砷脂，特别是含砷碳氢化合物 (AsHC)，是一类新兴的海鲜污染物。在这里，我们对培养的人肝 (HepG2) 细胞中最近鉴定的 oxo-AsHC 332 代谢物 thioxo-AsHC 348 进行毒理学表征。与母体化合物 oxo-AsHC 332 的先前研究结果相比，硫代-AsHC 348 在相同浓度范围内显着影响细胞活力，但细胞生物利用度降低约 10 倍。与oxo-AsHC 332类似，thioxo-AsHC 348基本上不会诱导氧化应激或DNA损伤。此外，与 oxo-AsHC 相比，332 线粒体似乎不是 thioxo-AsHC 的主要亚细胞毒性靶标。这项研究表明，thioxo-AsHC 348 的毒性至少与其母体化合物 oxo-AsHC 332 一样，但很可能通过不同的毒性作用模式发挥作用，这一模式仍需鉴定。