The MinION sequencer by Oxford Nanopore Technologies turns DNA and RNA sequencing into a routine task in biology laboratories or in field research. For downstream analysis it is required to have a sufficient amount of target reads. Especially prokaryotic or bacteriophagic sequencing samples can contain a significant amount of off-target sequences in the processed sample, stemming from human DNA/RNA contamination, insufficient rRNA depletion, or remaining DNA/RNA from other organisms (e.g. host organism from bacteriophage cultivation). Such impurity, contamination and off-targets (ICOs) block read capacity, requiring to sequence deeper. In comparison to second-generation sequencing, MinION sequencing allows to reuse its chip after a (partial) run. This allows further usage of the same chip with more sample, even after adjusting the library preparation to reduce ICOs. The earlier a sample’s ICOs are detected, the better the sequencing chip can be conserved for future use. Here we present sequ-into, a low-resource and user-friendly cross-platform tool to detect ICO sequences from a predefined ICO database in samples early during a MinION sequencing run. The data provided by sequ-into empowers the user to quickly take action to preserve sample material and chip capacity. sequ-into is available from https://github.com/mjoppich/sequ-into

Oxford Nanopore Technologies 的 MinION 测序仪将 DNA 和 RNA 测序变成生物实验室或现场研究的常规任务。对于下游分析，需要有足够数量的目标读数。特别是原核或噬菌体测序样品在处理的样品中可能含有大量脱靶序列，这些脱靶序列源于人类 DNA/RNA 污染、rRNA 去除不足或来自其他生物体（例如来自噬菌体培养的宿主生物体）的剩余 DNA/RNA。此类杂质、污染和脱靶 (ICO) 会阻碍读取能力，从而需要进行更深层次的测序。与第二代测序相比，MinION 测序允许在（部分）运行后重复使用其芯片。即使在调整文库制备以减少 ICO 之后，这也允许进一步使用具有更多样本的同一芯片。越早检测到样品的 ICO，就能更好地保存测序芯片以供将来使用。在这里，我们介绍了sequ-into，这是一种资源匮乏且用户友好的跨平台工具，用于在 MinION 测序运行早期从样本中的预定义 ICO 数据库中检测 ICO 序列。sequ-into 提供的数据使用户能够快速采取行动以保护样品材料和芯片容量。sequ-into 可从 https://github.com/mjoppich/sequ-into 获取