In this study, a rapid, stable, and sensitive colorimetric/surface-enhanced Raman spectroscopy (SERS) method was developed based on a polyvinyl alcohol‐decorated Au nanoparticle (PVA–Au) colloid for the in situ detection of thiram residues in fruits and vegetables. The PVA–Au colloid acted as both a colorimetric and SERS sensor. The colorimetric detection of thiram is based on the color change and absorption band shift caused by aggregation of the PVA–Au colloid. The stable and controllable colorimetric process has been verified. The influence of the media pH and reaction time on the aggregation of the PVA–Au colloid were also investigated. The reflectance and SERS spectra of the thiram samples were acquired and analyzed. The limit of the colorimetric detection of thiram in apples, pears, and vegetables was 4.62, 7.83, and 10.74 ppb ( μ g kg −1 ), respectively, which is well below the maximum residue level of 5 ppm (mg kg −1 ) set by the National Foo...

中文翻译：

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比色法/表面增强拉曼光谱法原位检测水果和蔬菜中的锡拉姆

在这项研究中，开发了一种基于聚乙烯醇修饰的金纳米粒子（PVA-Au）胶体的快速，稳定和灵敏的比色/表面增强拉曼光谱（SERS）方法，用于原位检测水果和水果中的锡兰残留物。蔬菜。PVA-Au胶体同时用作比色和SERS传感器。锡兰的比色检测是基于PVA-Au胶体聚集引起的颜色变化和吸收带位移。比色法稳定且可控。还研究了培养基pH和反应时间对PVA-Au胶体聚集的影响。采集并分析了硫氰酸盐样品的反射率和SERS光谱。比色法检测苹果，梨和蔬菜中锡拉姆的限度为4.62、7.83和10.74 ppb（μg kg -1），