Induced pluripotent stem (iPS) cells have been produced just for a few species among order Carnivora: snow leopard, Bengal tiger, serval, jaguar, cat, dog, ferret, and American mink. We applied the iPS cell derivation protocol to the ringed seal (Phoca hispida) fibroblasts. The resulting cell line had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed us to register expression of AFP, endoderm marker, and Cdx2, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably are not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipo- and osteocytes.

中文翻译：

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环斑海豹（Phoca hispida）诱导的多能干细胞的衍生

食肉目中只为几种物种生产了诱导多能干（iPS）细胞：雪豹，孟加拉虎、,、美洲虎，猫，狗，雪貂和美洲貂。我们将iPS细胞衍生协议应用于环状海豹（Phoca hispida）成纤维细胞。所得细胞系具有多能性标记基因Rex1的表达。胚状体样结构的差异使我们能够注册AFP（内胚层标志物）和Cdx2（滋养外胚层标志物）的表达，但不能记录神经元（外胚层）标志物的表达。这些细胞很容易分化为脂肪细胞和骨细胞，这是中胚层细胞的起源。转录组分析使我们可以得出结论，该细胞系与人多能细胞不相似，因此，很可能不是多能细胞。因此，