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Bioassay screening of 12 Iranian plants and detection of antibacterial compounds from Heracleum persicum using a TLC bioautography method
Journal of Liquid Chromatography & Related Technologies ( IF 1.0 ) Pub Date : 2020-05-23 , DOI: 10.1080/10826076.2020.1725557
Hossein Dehghan 1 , Parizad Rezaee 2 , Atousa Aliahmadi 2
Affiliation  

Abstract Allium paradoxum, Buxus hyrcana, Convolvulus persicus, Cyclamen coum, Eryngium caucasicum, Heracleum persicum, Pimpinella affinis, Parrotia persica, Primula heterochroma, Pyrus boissieriana, Ruscus hyrcanus, and Smilax excelsa are some medicinal and food flavoring plants found in the Hyrcanian forests in Iran. This study was designed to determine the most potent antibacterial compounds from the plants. The MIC values of n-hexane, ethyl acetate, and methanol extracts of different parts of the plants (63 extracts) were determined against Staphylococcus aureus, B. cereus, Pseudomonas aeruginosa, and Salmonella entritidis. The antibacterial compounds of the most potent extracts were determined by TLC bioautography, against S. aureus. According to the results, the n-hexane extract of the roots of H. persicum was selected to identify its bioactive phytochemicals. In the following, eight furanocoumarins, heratomin, xanthotoxin, isobergapten, bergapten, pimpinellin, isopimpinellin, moellendorffilin, and sphondin were identified by comparing with purified standards in different solvent systems. Among these compounds, moellendorffilin played a dominant role and exhibited significant activities against S. aureus, B. cereus, P. aeruginosa, and S. entritidis, with MIC values 31.25, 31.25, 62.5, and 15.62 µg/mL, respectively. Accordingly, H. persicum could be regarded as a promising high-potential plant and inexpensive source of antibacterial compounds for the pharmaceutical and food industries. Graphical Abstract

中文翻译:

使用薄层色谱生物自显影法对 12 种伊朗植物进行生物测定筛选并检测来自 Heracleum persicum 的抗菌化合物

摘要 葱属、黄杨、白花旋花、仙客来、刺槐、白花仙草、紫菖蒲、百合、报春花、梨属、黑木耳、仙客来、仙客来是一些药用和食用香料植物。伊朗。本研究旨在确定植物中最有效的抗菌化合物。测定了植物不同部位的正己烷、乙酸乙酯和甲醇提取物(63 种提取物)对金黄色葡萄球菌、蜡状芽孢杆菌、铜绿假单胞菌和沙门氏菌的 MIC 值。最有效提取物的抗菌化合物通过 TLC 生物自显影法测定,针对金黄色葡萄球菌。根据结果​​,H.根的正己烷提取物。选择桃来鉴定其生物活性植物化学物质。下面通过与不同溶剂体系中的纯化标准品进行比较,鉴定了八种呋喃香豆素、heratomin、黄酮素、异佛手柑、佛手柑、pimpinellin、isopimpinellin、moellendorffilin和sphondin。在这些化合物中,moellendorffilin 发挥了主导作用,对金黄色葡萄球菌、蜡状芽孢杆菌、铜绿假单胞菌和 S.entritidis 表现出显着的活性,MIC 值分别为 31.25、31.25、62.5 和 15.62 µg/mL。因此,H. persicum 可被视为一种有前途的高潜力植物和制药和食品工业抗菌化合物的廉价来源。图形概要 moellendorffilin 和 sphondin 通过与不同溶剂系统中的纯化标准进行比较来鉴定。在这些化合物中,moellendorffilin 发挥了主导作用,对金黄色葡萄球菌、蜡状芽孢杆菌、铜绿假单胞菌和 S.entritidis 表现出显着的活性,MIC 值分别为 31.25、31.25、62.5 和 15.62 µg/mL。因此,H. persicum 可被视为一种有前途的高潜力植物和制药和食品工业抗菌化合物的廉价来源。图形概要 moellendorffilin 和 sphondin 通过与不同溶剂系统中的纯化标准进行比较来鉴定。在这些化合物中,moellendorffilin 发挥了主导作用,对金黄色葡萄球菌、蜡状芽孢杆菌、铜绿假单胞菌和 S.entritidis 表现出显着的活性,MIC 值分别为 31.25、31.25、62.5 和 15.62 µg/mL。因此,H. persicum 可被视为一种有前途的高潜力植物和制药和食品工业抗菌化合物的廉价来源。图形概要 分别为 62 µg/mL。因此,H. persicum 可被视为一种有前途的高潜力植物和制药和食品工业抗菌化合物的廉价来源。图形概要 分别为 62 µg/mL。因此,H. persicum 可被视为一种有前途的高潜力植物和制药和食品工业抗菌化合物的廉价来源。图形概要
更新日期:2020-05-23
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