Pyraoxystrobin is a novel strobilurin fungicide that is widely used on many crops. The high log Kow of pyraoxystrobin implies that it tends to accumulate in aquatic organisms. This study optimized the sorbents of QuEChERS (quick, easy, cheap, effective, rugged, and safe) using 13C-labelled pyraoxystrobin as the internal standard (IS). It has been established a QuEChERS-LC-MS/MS IS method to study the bioconcentration and elimination of pyraoxystrobin in zebrafish (Danio rerio). The results indicated that the method had satisfactory linearity between 0.234 and 15 μg L-1 (R2 = 0.9996). The limits of detection (LOD) and quantification (LOQ) for pyraoxystrobin were 0.01 and 0.03 μg L-1, respectively. The LOQs of the method for water and zebrafish were 0.05 μg L-1 and 0.01 mg/kg, respectively. The mean recovery of pyraoxystrobin in zebrafish and water at fortification levels of 0.01-0.3 mg kg-1 and 0.05-1.5 μg L-1 ranged from 98.31 to 105.61% and 101.87 to 108.48%, respectively, with a % RSD (relative standard deviation) of 0.94-3.57%. The bioconcentration has been evaluated. The bioconcentration factors for pyraoxystrobin in zebrafish were 1,792 and 3,505 after exposure to 0.5 μg L-1 for 168 h and 0.05 μg L-1 for 216 h, respectively. The half-life of pyraoxystrobin in zebrafish was 9.0-9.5 d.

中文翻译：

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使用改良的QuEChERS提取方法评估斑马鱼（Danio rerio）中吡菌酯的生物浓度。

吡喃木酚酯是一种新的反伞花绿素杀真菌剂，已广泛用于许多农作物。吡菌酯的高log Kow值表明它倾向于在水生生物中积累。这项研究使用13C标记的吡咯菌酯作为内标（IS）优化了QuEChERS的吸附剂（快速，简便，便宜，有效，坚固和安全）。已经建立了QuEChERS-LC-MS / MS IS方法来研究斑马鱼（Danio rerio）中吡菌酯的生物浓度和消除。结果表明，该方法在0.234和15μgL-1之间具有令人满意的线性（R2 = 0.9996）。吡菌酯的检测限（LOD）和定量限（LOQ）分别为0.01和0.03μgL-1。该方法对水和斑马鱼的最低定量限分别为0.05μgL-1和0.01 mg / kg。设防浓度为0.01-0.3 mg kg-1和0.05-1.5μgL-1时，斑马鱼和水中吡菌酯的平均回收率分别为98.31％至105.61％和101.87％至108.48％，相对标准偏差为％（相对标准偏差） ）0.94-3.57％。已经评估了生物浓度。分别暴露于0.5μgL-1 168 h和0.05μgL-1 216 h后，斑马鱼中吡菌酯的生物浓缩因子分别为1,792和3,505。吡菌酯在斑马鱼中的半衰期为9.0-9.5 d。5μgL-1持续168 h和0.05μgL-1持续216 h。吡菌酯在斑马鱼中的半衰期为9.0-9.5 d。5μgL-1持续168 h和0.05μgL-1持续216 h。吡菌酯在斑马鱼中的半衰期为9.0-9.5 d。