Bioscience, Biotechnology, and Biochemistry ( IF 1.4 ) Pub Date : 2020-05-24 , DOI: 10.1080/09168451.2020.1770572 Hock Wei Tang 1 , Sahar Abbasiliasi 2 , Paramasivam Murugan 1 , Yew Joon Tam 3 , Hui Suan Ng 4 , Joo Shun Tan 1
The aims of this study were to compare the effectiveness of different drying methods and to investigate the effects of adding a series of individual protectant such as skim milk, sucrose, maltodextrin, and corn starch for preserving Lactobacillus acidophilus FTDC 3081 cells during spray and freeze-drying and storage at different temperatures. Results showed a remarkable high survival rate of 70–80% immediately after spray- and freeze-drying in which the cell viability retained at the range of 109 to 1010 CFU/mL. After a month of storage, maltodextrin showed higher protective ability on both spray- and freeze-dried cells as compared to other protective agents at 4°C, 25°C, and 40°C. A complete loss in viability of spray-dried L. acidophilus FTDC 3081 was observed after a month at 40°C in the absence of protective agent.
中文翻译:
冷冻干燥和喷雾干燥保存方法对不同类型封装的嗜酸乳杆菌FTDC 3081保护剂存活率的影响。
这项研究的目的是比较不同干燥方法的效果,并研究添加一系列单独保护剂(如脱脂牛奶,蔗糖,麦芽糖糊精和玉米淀粉)在喷雾和冷冻过程中保存嗜酸乳杆菌FTDC 3081细胞的效果。在不同温度下干燥和储存。结果显示,喷雾干燥和冷冻干燥后,立即存活率高达70-80%,细胞存活率保持在10 9至10 10 CFU / mL范围内。储存一个月后,与其他在4°C,25°C和40°C的保护剂相比,麦芽糖糊精对喷雾和冻干细胞均显示出更高的保护能力。喷雾干燥的生命力完全丧失 在没有保护剂的情况下于40°C放置一个月后观察到嗜酸乳杆菌FTDC 3081。