In this study, magnetic Fe₃O₄ nanoparticles coated with polydopamine possessing abundant amino groups (Fe₃O₄@PDA) were conveniently prepared, detailed, and characterized, and then firstly used as a supporting matrix for immobilizing Thermomyces lanuginosus lipase (Fe₃O₄@). The effects of some crucial factors on the immobilization efficiency were investigated and the optimal protein loading and activity recovery were found to be 156.4 mg/g and 90.9%, respectively. Characterization studies revealed that Fe₃O₄@[email protected] displayed a broader pH and temperature adaptability as compared to the free TLL, which allows its use at wider ranges of reaction conditions. With regard to the stabilities, the immobilized TLL clearly displayed improved pH, thermal, and solvent tolerance stabilities compared to the free enzyme, suggesting that the biocompatible Fe₃O₄@PDA might be an outstanding material for immobilizing TLL and acting as alternative support for different enzymes. View Full-Text

中文翻译：

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固定在新型聚多巴胺功能的磁性Fe3O4纳米粒子上的嗜热单胞菌脂肪酶的催化性能的改善。

在本研究中，方便地制备，详细描述和表征了涂覆有丰富氨基的聚多巴胺的磁性Fe ₃ O ₄纳米颗粒（Fe ₃ O ₄ @PDA），然后首先将其用作固定嗜热单胞菌脂肪酶（Fe ₃ O ₄ @）。研究了一些关键因素对固定化效率的影响，发现最佳蛋白质负载量和活性恢复分别为156.4 mg / g和90.9％。表征研究表明，Fe ₃ O ₄与免费的TLL相比，@ [电子邮件保护的]显示出更宽的pH和温度适应性，这使其可以在更宽的反应条件范围内使用。关于稳定性，固定化TLL与游离酶相比明显显示出改善的pH，热和溶剂耐受性稳定性，表明生物相容性Fe ₃ O ₄ @PDA可能是固定化TLL的出色材料，并可以作为替代性支持不同的酶。查看全文