Melanoma is one of the most serious skin cancers. The incidence of this malignant skin lesion is continuing to increase worldwide. Melanoma is resistant to chemotherapeutic drugs and highly metastatic. Surgical resection can only be used to treat melanoma in the early stages, while chemotherapy is limited due to melanoma multi-drug resistance. The overexpression of glutathione S-transferase (GST) may have a critical role in this resistance. Caffeic acid phenethyl ester (CAPE) is a natural phenolic compound, which occurs in many plants. Previous studies demonstrated that CAPE suppresses the growth of melanoma cells and induces reactive oxygen species generation. It is also known that bioactivation of CAPE to its corresponding quinone metabolite by tyrosinase would lead to GST inhibition and selective melanoma cell death. We investigated the biochemical toxicity of CAPE in combination with microsecond electropermeabilization in two human melanoma cell lines. Our results indicate that electroporation of melanoma cells in the presence of CAPE induced high oxidative stress, which correlates with high cytotoxicity. Moreover, it can disrupt the metabolism of cancer cells by inducing apoptotic cell death. Electroporation of melanoma cells may be an efficient CAPE delivery system, enabling the application of this compound, while reducing its dose and exposure time.

中文翻译：

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可逆电穿孔辅助人黑素瘤细胞研究咖啡酸苯乙基酯。

黑色素瘤是最严重的皮肤癌之一。在世界范围内，这种恶性皮肤病变的发生率持续增加。黑色素瘤对化疗药物具有抵抗力，并且具有高度转移性。手术切除只能用于早期阶段的黑色素瘤，而化学疗法由于黑色素瘤的多重耐药性而受到限制。谷胱甘肽S的过表达-转移酶（GST）可能在这种抗性中起关键作用。咖啡酸苯乙酯（CAPE）是一种天然酚类化合物，存在于许多植物中。先前的研究表明，CAPE抑制黑素瘤细胞的生长并诱导产生活性氧。还已知通过酪氨酸酶将CAPE生物活化为其相应的醌代谢物将导致GST抑制和选择性黑素瘤细胞死亡。我们调查了CAPE结合微秒电通透性在两种人黑素瘤细胞系中的生化毒性。我们的结果表明，在CAPE存在下，黑素瘤细胞的电穿孔诱导了高氧化应激，这与高细胞毒性有关。而且，它可以通过诱导凋亡细胞死亡来破坏癌细胞的代谢。