Here, we report an increase in biomass yield and saccharification in transgenic tobacco plants (Nicotiana tabacum L.) overexpressing thermostable β-glucosidase from Thermotoga maritima, BglB, targeted to the chloroplasts and vacuoles. The transgenic tobacco plants showed phenotypic characteristics that were significantly different from those of the wild-type plants. The biomass yield and life cycle (from germination to flowering and harvest) of the transgenic tobacco plants overexpressing BglB were 52% higher and 36% shorter than those of the wild-type tobacco plants, respectively, indicating a change in the genome transcription levels in the transgenic tobacco plants. Saccharification in biomass samples from the transgenic tobacco plants was 92% higher than that in biomass samples from the wild-type tobacco plants. The transgenic tobacco plants required a total investment (US$/year) corresponding to 52.9% of that required for the wild-type tobacco plants, but the total biomass yield (kg/year) of the transgenic tobacco plants was 43% higher than that of the wild-type tobacco plants. This approach could be applied to other plants to increase biomass yields and overproduce β-glucosidase for lignocellulose conversion.

中文翻译：

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转基因烟草过表达β-葡萄糖苷酶的生物量提高和糖化作用。

在这里，我们报告了过量表达转基因烟草植物（Nicotiana tabacum L.）的生物量产量和糖化作用的增加，该植物过度表达了热稳定的β-葡萄糖苷酶（来自巴西嗜热菌，BglB，针对叶绿体和液泡）。转基因烟草植物表现出与野生型植物显着不同的表型特征。过表达BglB的转基因烟草植物的生物量产量和生命周期（从发芽到开花和收获）分别比野生型烟草植物高52％和短36％，表明转基因烟草植物中基因组转录水平的变化。转基因烟草植物的生物量样品中的糖化比野生型烟草植物的生物量样品中的糖化高92％。转基因烟草植物的总投资（美元/年）相当于野生型烟草植物的52.9％，但转基因烟草植物的总生物量产量（kg /年）比其高43％。野生型烟草植物。该方法可应用于其他植物以增加生物量产量并过量生产用于木质纤维素转化的β-葡萄糖苷酶。