The roles of phenolics from olive oils as effective anticancer agents have been documented in various in vitro studies of different cancer cells lines, but the relationship between the phenolic profile of olive oil and its biological activity needs more elucidation. In this study, we analysed phenolic profiles of extra virgin olive oils (EVOOs) from different autochthonous cultivars from Croatia (Oblica, Bjelica, Buža, Žižolera) and investigated the biological effect of EVOO phenolic extracts (EVOO-PEs) on human cervical (HeLa) and human colon (SW48) cancer cell lines alone and in combination with cisplatin (cDDP), carboplatin (CBP), 5-fluorouracil (5-FU) and irinotecan. The quantitative evaluation of olive oil polyphenols was performed by HPLC-DAD and spectrophotometric analysis. The biological effect of EVOO-PEs alone and in combination with anticancer drugs was measured by MTT assay. Analysed EVOO-PEs differ in phenolic profile and inhibited HeLa and SW48 cells in a dose-dependent manner. Further, it is shown that EVOO-PEs (Oblica-Sea, Buža and Žižolera), in combination with anticancer drugs, increase the metabolic activity of HeLa and SW48 cells and have a protective role. These data imply careful consummation of olive oil during chemotherapy of cancer patients.

中文翻译：

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特级初榨橄榄油中酚类化合物的生物活性，通过其酚类特征及其与在人宫颈癌和结肠腺癌细胞中观察到的抗癌药物的组合。


橄榄油中的酚类物质作为有效抗癌剂的作用已在不同癌细胞系的各种体外研究中得到证实，但橄榄油的酚类物质与其生物活性之间的关系需要更多阐明。在这项研究中，我们分析了来自克罗地亚不同本土品种（Oblica、Bjelica、Buža、Žizolera）的特级初榨橄榄油（EVOO）的酚类成分，并研究了 EVOO 酚类提取物（EVOO-PE）对人类宫颈（HeLa）的生物效应。 ）和人结肠（SW48）癌细胞系单独使用以及与顺铂（cDDP）、卡铂（CBP）、5-氟尿嘧啶（5-FU）和伊立替康联合使用。采用HPLC-DAD和分光光度分析对橄榄油多酚进行定量评价。通过MTT法测定了EVOO-PE单独使用以及与抗癌药物联合使用的生物学效应。分析的 EVOO-PE 酚类成分不同，并以剂量​​依赖性方式抑制 HeLa 和 SW48 细胞。此外，研究表明，EVOO-PE（Oblica-Sea、Buža 和 Žizolera）与抗癌药物联合使用，可增加 HeLa 和 SW48 细胞的代谢活性，并具有保护作用。这些数据意味着癌症患者化疗期间要谨慎使用橄榄油。