The Ca2+ -regulated protein kinase CIPK1 integrates plant responses to phosphate deficiency in Arabidopsis thaliana.,Plant Biology

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The Ca2+ -regulated protein kinase CIPK1 integrates plant responses to phosphate deficiency in Arabidopsis thaliana.
Plant Biology ( IF 4.2 ) Pub Date : 2020-05-23 , DOI: 10.1111/plb.13137
X Lu ₁ , X Li ₁ , D Xie ₁ , C Jiang ₂ , C Wang ₁ , L Li ₁ , Y Zhang ₁ , H Tian ₂ , H Gao ₁ , C Wang ₁

Affiliation

Phosphate (Pi) deficiency severely restricts plant growth and development, as Pi is an essential macronutrient. Calcium (Ca²⁺) is a ubiquitous second messenger in plants; calcineurin B‐like proteins (CBL) and CBL‐interacting protein kinases (CIPK) are signalling pathways that act as an important Ca²⁺ signalling network which integrates plants to fine tune the response to stress; however, whether CIPK are involved in Pi deficiency stress remains largely unknown.
In this study, we carried out a reverse genetic strategy to screen T‐DNA insertion mutants of CIPK isoforms under Pi deficiency in Arabidopsis thaliana. Then Pi content, transcription of phosphate starvation‐induced (PSI) genes, acid phosphatase activity and hydrogen peroxide were determined in the wild‐type (WT) and cipk1 mutant, respectively. The phenotype of CIPK1 complementation lines was analysed.
The cipk1 mutant had a more sensitive phenotype, with lower root elongation and root length, and decreased Pi content compared with the WT under Pi deficiency. Moreover, CIPK1 mutation caused phosphate starvation‐induced (PSI) genes to be significantly induced under Pi deficiency. Histological staining demonstrated that the cipk1 mutant had increased acid phosphatase activity and hydrogen peroxide concentration under Pi deficiency. By using the yeast two‐hybrid system, we further demonstrated the interaction between CIPK1 and the WRKY transcription factors, WRKY6 and WRKY42.
Overall, we demonstrate that CIPK1 is involved in the Pi deficiency signalling pathway in A. thaliana, revealing the important role of Ca²⁺ in the Pi nutrition signalling pathway, and potentially providing a theoretical foundation for molecular breeding of crops with better Pi utilization efficiency.

中文翻译：

Ca2 +调节的蛋白激酶CIPK1整合了植物对拟南芥中磷酸盐缺乏的反应。

磷（Pi）缺乏严重限制了植物的生长和发育，因为Pi是必需的大量营养素。钙（Ca ²⁺）是植物中普遍存在的第二种信使。钙调神经磷酸酶B样蛋白（CBL）和CBL相互作用蛋白激酶（CIPK）是重要的Ca ²⁺信号传导网络，它整合了植物以微调对胁迫的响应。然而，CIPK是否参与了Pi缺乏胁迫尚不清楚。
在这项研究中，我们进行了逆向遗传策略，以筛选拟南芥中Pi缺失下CIPK亚型的T-DNA插入突变体。然后分别测定野生型（WT）和cipk1突变体中的Pi含量，磷酸饥饿诱导（PSI）基因的转录，酸性磷酸酶活性和过氧化氢。分析了CIPK1互补系的表型。
与缺磷的WT相比，cipk1突变体具有更高的表型，较低的根伸长和根长，且磷含量降低。此外，CIPK1突变导致磷缺乏引起磷饥饿诱导（PSI）基因的显着诱导。组织学染色表明，在Pi缺乏下，cipk1突变体具有增加的酸性磷酸酶活性和过氧化氢浓度。通过使用酵母双杂交系统，我们进一步证明了CIPK1与WRKY转录因子WRKY6和WRKY42之间的相互作用。
总的来说，我们证明CIPK1参与了拟南芥中的Pi缺乏信号传导途径，揭示了Ca ²⁺在Pi营养信号传导途径中的重要作用，并可能为Pi利用率更高的作物分子育种提供理论基础。。

更新日期：2020-05-23

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