Colonization and maintenance of mosquitoes in the laboratory is required to study physiology, ecology, and behavior of mosquitoes and interactions between mosquito and pathogens. Artificial blood feeding systems have been widely used to maintain the laboratory colony of Aedes albopictus. In this study, we investigated the effects of mating period (1, 3, 6, and 10 days) and time‐of‐day for bloodmeal (08:00, 13:00, and 18:00) in the use of an artificial feeding system on blood‐feeding rate, female fecundity, egg hatching rate, and developmental time of the Asian tiger mosquito, A. albopictus. Younger females mated for three or fewer days reproduced more eggs compared to those of oldest females mated for ten days. Similar to the result for eggs laid, the mean egg‐hatching rate was significantly higher from the offspring of younger females than from those of older females. However, mating period and time‐of‐day for bloodmeal had no effect on the blood feeding rate and developmental time. Taken together, we suggest that three‐day mating with bloodmeal at 18:00 is optimal for maintaining colonies of A. albopictus in laboratory conditions.

中文翻译：

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实验室条件下血粉交配期和日间时间对亚洲虎蚊（白纹伊蚊）饲养的影响

需要在实验室中定殖和维护蚊子，以研究蚊子的生理、生态和行为以及蚊子与病原体之间的相互作用。人工采血系统已被广泛用于维持白纹伊蚊的实验室群体。在这项研究中，我们调查了交配期（1、3、6 和 10 天）和血粉的一天中的时间（08:00、13:00 和 18:00）在使用人工摄食系统对亚洲虎蚊 A. albopictus 的吸血率、雌性繁殖力、卵孵化率和发育时间的影响。与交配 10 天的最年长雌性相比，交配三天或更少的年轻雌性繁殖出更多的卵。与产卵的结果相似，年轻雌性后代的平均孵化率明显高于老年雌性后代。然而，血粉的交配期和一天中的时间对血饲率和发育时间没有影响。总之，我们建议在 18:00 与血粉交配三天是在实验室条件下维持白纹线虫菌落的最佳选择。