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Morphological analysis of aqueous humor drainage using QD nanoparticles and indocyanine green.
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-05-23 , DOI: 10.1002/jemt.23494
İsmail Hakkı Nur 1 , Şerife Saçmacı 2 , İmdat Orhan 1 , William Perez 3 , Haluk Testereci 4
Affiliation  

This study represents the first morphological description of the lymphatic drainage of the ciliary body in vivo by comparative hyperspectral fluorescence imaging techniques of Quantum Qdot655 (QD) nanoparticles and indocyanine green (ICG). A volume of 1.25 μl of QD was injected into the left anterior camera of all rats. Similarly,1.25 μl of ICG diluted at a ratio of one‐fourth with physiological saline solution was injected into the right anterior camera of all rats. The thickness of the skin in the mandibular area, connective tissue, and the depth of the mandibular lymph node (MLN) made image retrieval difficult. For QD, 302 nm UV excitation and 605 nm fluorescence peak emission were applied. The detection of QD and ICG used in this study in the MLNs is definitive evidence that aqueous humor (AH) follows a uveolymphatic pathway. Scanning electron microscope and the energy dispersive X‐ray analyzer spectrum were used to examine both the Schlemm's canal and the MLN. For the first time, the QD was detected in the cortex of MLN. The QCM analysis of both QD‐AH and ICG‐AH was used to determine whether there was any interaction between them. This comparative study shows the importance of experimental animal modeling in pharmacological studies regarding eye research and drugs. In a female rat, the signal was taken from the parotid lymph node with QD injections.

中文翻译:

QD纳米颗粒和吲哚菁绿对房水引流的形态学分析。

这项研究代表了通过量子Qdot655(QD)纳米颗粒和吲哚菁绿(ICG)的比较高光谱荧光成像技术对体内睫状体淋巴引流进行的第一个形态学描述。将1.25μlQD体积注入所有大鼠的左前摄像头。同样,将1.25μl用生理盐水稀释四分之一的ICG注入所有大鼠的右前摄像头。下颌区域的皮肤厚度,结缔组织以及下颌淋巴结(MLN)的深度使图像检索变得困难。对于QD,应用302 nm紫外激发和605 nm荧光峰发射。在MLN中用于本研究的QD和ICG的检测是明确的证据,房水(AH)遵循淋巴淋巴途径。扫描电子显微镜和能量色散X射线分析仪光谱用于检查Schlemm根管和MLN。首次在MLN皮质中检测到QD。QD-AH和ICG-AH的QCM分析用于确定它们之间是否存在任何相互作用。这项比较研究表明,在有关眼科研究和药物的药理研究中,实验动物模型的重要性。在雌性大鼠中,信号是通过QD注射从腮腺淋巴结获取的。这项比较研究表明,在有关眼科研究和药物的药理研究中,实验动物建模的重要性。在雌性大鼠中,该信号是通过QD注射从腮腺淋巴结获取的。这项比较研究表明,在有关眼科研究和药物的药理研究中,实验动物建模的重要性。在雌性大鼠中,信号是通过QD注射从腮腺淋巴结获取的。
更新日期:2020-05-23
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