Resistin as an adipokine identified from rodents in 2001 is involved in many biological process. However, little is known about this gene in fish. We cloned Siberian sturgeon (Acipenser baerii) resistin cDNA of 795 base pairs, encoding 107 amino acids, which showed 38%-40% identity to human and rodents. Real-time quantitative PCR showed that resistin gene was widely distributed in tissues of Siberian sturgeon, with the highest expression in liver. After fasting for 1, 3, 6 and 10 days, the expression of resistin gene in the liver of Siberian sturgeon decreased significantly, and after re-feeding on the 10 days of fasting the resistin mRNA expression increased rapidly, suggesting that resistin may play an important role in liver in response to starvation. Taken together, these results suggest that resistin may be involved in the regulation of energy homeostasis in liver.

中文翻译：

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西伯利亚鲟(Acipenser baerii)抵抗素基因的表征、组织分布及禁食和再喂养对抵抗素mRNA表达的影响

抵抗素是2001年从啮齿类动物中鉴定出的一种脂肪因子，参与了许多生物过程。然而，人们对鱼类中的这种基因知之甚少。我们克隆了 795 个碱基对的西伯利亚鲟 (Acipenser baerii) 抵抗素 cDNA，编码 107 个氨基酸，与人类和啮齿动物显示 38%-40% 的同一性。实时定量PCR显示，抵抗素基因广泛分布于鲟鱼组织中，以肝脏表达量最高。禁食1、3、6、10天后，西伯利亚鲟肝脏中抵抗素基因的表达显着降低，禁食10天后重新进食后抵抗素mRNA表达迅速增加，提示抵抗素可能发挥重要作用。在肝脏对饥饿的反应中起重要作用。综合起来，