Acinetobacter baumannii , one of the most life‐threatening nosocomial drug‐resistant pathogens, imposes high morbidity and mortality rates, thus highlighting immunization‐based treatments or prevention measures. The selection of appropriate antigens can elicit protective immunity. The gene encoding a fimbrial protein introduced via reverse vaccinology was cloned, expressed and evaluated for immunogenicity in a murine model. Mice immunized with the recombinant protein were challenged with A. baumannii ATCC 19606. Adherence to A549 cell line of specific anti‐sera treated A. baumannii was also assessed. Passive immunity was evaluated in a murine pneumonia model. Indirect ELISA showed a high specific antibody titre. Adherence of A. baumannii to A549 cell line decreased by 40% after incubation with 1:250 dilution of specific anti‐sera. All the actively immunized mice survived. Bacterial load in the spleen and liver of the immunized mice was 3‐fold lower than those of the control. The number of bacteria in the lungs of passively immunized mice was about 6‐fold lower than the control mice. The fimbrial protein could be considered as a promising protective immunogen against A. baumannii .

中文翻译：

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用保守的免疫原性纤维蛋白预防医院鲍曼不动杆菌感染。

鲍曼不动杆菌是最致命的医院内耐药性病原体之一，其发病率和死亡率很高，因此强调了基于免疫的治疗或预防措施。适当抗原的选择可以引起保护性免疫。克隆，表达并通过鼠疫模型评估了通过反向疫苗学引入的编码纤维蛋白的基因。用重组蛋白免疫的小鼠用鲍曼不动杆菌ATCC 19606攻击。还评估了特定抗血清处理的鲍曼不动杆菌对A549细胞系的粘附性。在鼠肺炎模型中评估了被动免疫。间接ELISA显示高特异性抗体滴度。鲍曼不动杆菌的坚持用特异性抗血清的1：250稀释液孵育后，A549细胞的A549细胞下降40％。所有主动免疫的小鼠均存活。免疫小鼠的脾脏和肝脏中的细菌负荷比对照组低3倍。被动免疫小鼠肺中的细菌数量比对照小鼠低约6倍。纤维蛋白可以被认为是针对鲍曼不动杆菌的有希望的保护性免疫原。