Lysogenic bacterial strains abound in the Lactobacillus genus and contain dormant prophages inserted within their genomes. To evaluate the prophage-induction potential of the Lactobacillus strains of six species, 142 randomly selected strains from these species were induced with Mitomycin C. Eight newly-induced phages were identified and found to be diverse in morphology. Among the six species assessed, Lactobacillus plantarum and Lactobacillus rhamnosus strains were generally insensitive to induction.

The genomic characterizations of eight phages were performed via whole genome sequencing and protein prediction. Meanwhile, genome comparison of the induced phages and predicted prophages demonstrated that the prediction software PHASTER can accurately locate major prophage regions in Lactobacillus. A phylogenetic tree of the Lactobacillus phage population was constructed to obtain further insights into the clustering of individuals, two major groups were found, one of which consisted mostly of L. plantarum virulent phages, the other was represented by Lactobacillus casei/paracasei temperate phages. Finally, it was confirmed via genomic collinear analysis, which seven of the eight Lactobacillus temperate phages were newly discovered, and two Lactobacillus brevis temperate phages belonged to a novel lineage.

溶菌性细菌菌株在乳杆菌属中比比皆是，并且含有插入其基因组中的休眠噬菌体。为了评估六个物种的乳杆菌菌株的前噬菌体诱导潜力，用丝裂霉素C诱导了从​​这些物种中随机选择的142个菌株。鉴定了八个新诱导的噬菌体，发现它们的形态各异。在所评估的六个物种中，植物乳杆菌和鼠李糖乳杆菌菌株通常对诱导不敏感。

通过全基因组测序和蛋白质预测对八个噬菌体进行了基因组表征。同时，对诱导的噬菌体和预测的噬菌体的基因组比较表明，预测软件PHASTER可以准确定位乳酸菌中的主要噬菌体区域。构建了乳杆菌噬菌体种群的系统树，以进一步了解个体的集群，发现了两个主要类别，其中一个主要由植物乳杆菌强毒噬菌体组成，另一个由干酪乳杆菌/副干酪温带菌噬菌体代表。最后，通过基因组共线性分析证实了八种乳酸杆菌中的七种新发现了温带噬菌体，并且两个短乳杆菌温带噬菌体属于新的谱系。