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SpRY: Engineered CRISPR/Cas9 Harnesses New Genome-Editing Power.
Trends in Genetics ( IF 13.6 ) Pub Date : 2020-05-23 , DOI: 10.1016/j.tig.2020.05.001
Dangquan Zhang 1 , Baohong Zhang 2
Affiliation  

Due to protospacer adjacent motif (PAM) requirements, CRISPR/Cas9 cannot access many genetic loci. A recent study by Walton et al. structurally engineered Streptococcus pyogenes Cas9 (SpCas9) to near-PAMless SpRY that can target most DNA sequences with high editing efficiency and flexibility. This newly engineered SpRY will potentially expand genome-editing capabilities for basic and applied research.



中文翻译:

SpRY:工程CRISPR / Cas9利用新的基因组编辑能力。

由于原生间隔子相邻基序(PAM)的要求,CRISPR / Cas9无法访问许多遗传基因座。Walton等人的最新研究结构化改造的化脓性链球菌Cas9(SpCas9)至近PAMless SpRY,可靶向大多数DNA序列,具有很高的编辑效率和灵活性。这个新设计的SpRY可能会扩展基因组编辑功能,用于基础研究和应用研究。

更新日期:2020-07-13
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