This study focused on identifying the rotenoids from the Tephrosia vogelli plant (fish-poison-bean), investigating the toxic potency of a crude T. vogelii extract and individual rotenoids (tephrosin, deguelin and rotenone) in vitro and in vivo and assessing the mode of action. A trout (Onychorynhis mykiss) gill epithelial cell line (RTgill-W1) was used to determine the cytotoxicity of rotenoids and effects on cell metabolism. Zebrafish (Danio rerio) aged from 3 h post fertilization (hpf) to 72 hpf were used for testing the developmental toxicity. The crude T. vogelii plant extract significantly decreased the cellular metabolic activity and was cytotoxic at lower concentrations (5 and 10 nM, respectively), while tephrosin, deguelin and rotenone showed these effects at concentrations ≥ 50 nM. The crude T. Vogelli extract had the highest toxic potency and induced adverse health effects in zebrafish including deformities and mortality at the lowest concentration (5 nM) compared to rotenone (10 nM) and deguelin and tephrosin (50 nM). These results indicate that the crude T. Vogelii extracts are highly potent and the bioactivity of these extracts warrant further investigation for their potential use to treat parasites in human and veterinary medicine and as a natural alternative to pesticides.

该研究集中于确定从所述鱼藤酮类灰叶vogelli植物（鱼毒豆），调查粗的毒性效力T. vogelii提取物和个体鱼藤酮类（灰叶素，鱼藤素和鱼藤酮）在体外和在体内和评估模式行动。用鳟鱼（Onychorynhis mykiss）g上皮细胞系（RTgill-W1）测定类胡萝卜素的细胞毒性及其对细胞代谢的影响。受精后3 h（hpf）至72 hpf的斑马鱼（Danio rerio）用于测试发育毒性。粗T. vogelii植物提取物显着降低了细胞的代谢活性，并且在较低浓度（分别为5和10 nM）下具有细胞毒性，而当浓度≥50 nM时，球蛋白，deguelin和鱼藤酮显示出这些作用。与鱼藤酮（10 nM）和杜格林和特罗芬（50 nM）相比，粗制的T. Vogelli提取物在斑马鱼中具有最高的毒性，并引起了不良健康影响，包括畸形和死亡率最低的浓度（5 nM）。这些结果表明，粗T. Vogelii提取物具有很强的效力，这些提取物的生物活性值得进一步研究，因为其潜在用途可用于治疗人类和兽医学中的寄生虫以及作为农药的天然替代品。