Abstract Context This is the first study on the antioxidant and anti-inflammatory activities of Leuzea conifera (Asteraceae). Objective The objective of this study is to evaluate in vitro the antioxidant and anti-inflammatory activities and to determine total phenols, total flavonoids content of different organic extracts of Leuzea conifera roots. Material and methods The in vitro antioxidant activity of the extracts were examined by three different methods, 2,2-diphenyl-1-picrylhydrazyl (DPPH), b-carotene and cupric reducing antioxidant capacity (CUPRAC). The in-vitro anti-inflammatory activity of Leuzea conifera was determined by protein denaturation method. Results In three methods tested to evaluate the antioxidant activity, ethyl acetate fraction displayed the highest antioxidant capacity (IC50 value: 23.26 ± 0.36 µg/mL in DPPH assay, IC50 value: 7.24 ± 0.25 µg/mL in β-Carotene-linoleic acid assay, and A0.50 value: 11.25 ± 0.27 µg/mL in CUPRAC). In this extract, the amount of total phenolics and total flavonoids were very high (344.98 ± 0.28 mg gallic acid equivalent/g, 186.94 ± 0.33 mg quercetin equivalent/g respectively). As for anti-inflammatory essay, ethyl acetate fraction has good activity (97.48 ± 0.68). Conclusion Organic extracts of Leuzea conifera roots showed better anti-oxidant and anti-inflammatory activity, essentially for the ethyl acetate fraction.

中文翻译：

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针叶树根的水醇提取物及其组分的抗氧化和抗炎活性的体外评价

摘要 背景本研究首次研究了针叶树(Leuzea conifera) (菊科) 的抗氧化和抗炎活性。目的本研究的目的是评价针叶树根不同有机提取物的体外抗氧化和抗炎活性，并测定总酚、总黄酮含量。材料和方法 提取物的体外抗氧化活性通过三种不同的方法检测，即 2,2-二苯基-1-苦基肼 (DPPH)、β-胡萝卜素和铜还原抗氧化能力 (CUPRAC)。通过蛋白质变性法测定了针叶树的体外抗炎活性。结果 在评估抗氧化活性的三种方法中，乙酸乙酯部分显示出最高的抗氧化能力（在 DPPH 测定中，IC50 值：23.26 ± 0.36 µg/mL，IC50 值：β-胡萝卜素-亚油酸测定中的 7.24 ± 0.25 µg/mL，A0.50 值：CUPRAC 中的 11.25 ± 0.27 µg/mL）。在该提取物中，总酚类和总黄酮的含量非常高（分别为 344.98 ± 0.28 mg 没食子酸当量/g、186.94 ± 0.33 mg 槲皮素当量/g）。对于抗炎文章，乙酸乙酯部分具有良好的活性（97.48±0.68）。结论 针叶树根的有机提取物显示出更好的抗氧化和抗炎活性，尤其是乙酸乙酯部分。乙酸乙酯馏分具有良好的活性（97.48±0.68）。结论 针叶树根的有机提取物显示出更好的抗氧化和抗炎活性，尤其是乙酸乙酯部分。乙酸乙酯馏分具有良好的活性（97.48±0.68）。结论 针叶树根的有机提取物显示出更好的抗氧化和抗炎活性，尤其是乙酸乙酯部分。