Former preterm infants, many of whom required supplemental O₂ support, exhibit sleep disordered breathing and attenuated ventilatory responses to acute hypoxia (HVR) beyond their NICU stay. There is an increasing awareness that early detection of biomarkers in biological fluids may be useful predictors/identifiers of short- and long-term morbidities. In the present study, we identified serotonin (5-HT), dopamine (DA) and hyaluronan (HA) as three potential biomarkers that may be increased by neonatal hyperoxia and tested whether they would be associated with an impaired HVR in a rat model of supplemental O₂ exposure. Neonatal rats (postnatal age (P) 6 days, P6) exposed to hyperoxia (40% FIO₂, 24 hrs/day between P1-P5 days of age) exhibited an attenuated early (1 minute), but not the late (4-5 minutes) phase of the HVR compared to normoxia control rats; the attenuated early phase HVR was associated with increased levels of DA (urine and serum), 5-HT (platelet poor plasma only, PPP), and HA (serum only). At P21, both the early and late phases of the HVR were attenuated, but serum and urine levels of all 3 biomarkers were similar to age-matched control rats. These data indicate that changes in several serum and/or urine biomarkers (5-HT, DA, and HA) following short-term (days) neonatal hyperoxia can signify long-term (weeks) respiratory control dysfunction. Further studies are needed to determine whether early detection of similar biomarkers could be convenient predictors of increased risk of abnormalities in respiratory control including sleep disordered breathing in former preterm infants who had received prior supplemental O₂ and who might also be at increased risk of SIDS.

_{许多需要补充 O 2}支持的早产儿在 NICU 停留后表现出睡眠呼吸紊乱和对急性缺氧 (HVR) 的通气反应减弱。人们越来越意识到生物体液中生物标志物的早期检测可能是短期和长期发病率的有用预测因子/识别因子。在本研究中，我们将血清素 (5-HT)、多巴胺 (DA) 和乙酰透明质酸 (HA) 确定为三种可能因新生儿高氧而增加的潜在生物标志物，并测试了它们是否与大鼠模型中的 HVR 受损有关补充 O ₂暴露。新生大鼠（出生后年龄（P）6天，P6）暴露于高氧（40% FIO ₂，P1-P5 日龄之间 24 小时/天）与常氧对照大鼠相比，HVR 早期（1 分钟）但未晚期（4-5 分钟）；减毒的早期 HVR 与 DA（尿液和血清）、5-HT（仅贫血小板血浆，PPP）和 HA（仅血清）水平升高有关。在 P21，HVR 的早期和晚期均减弱，但所有 3 种生物标志物的血清和尿液水平与年龄匹配的对照大鼠相似。这些数据表明，短期（数天）新生儿高氧后几种血清和/或尿液生物标志物（5-HT、DA 和 HA）的变化可能表示长期（数周）呼吸控制功能障碍。₂并且谁也可能处于增加的 SIDS 风险中。