The European eel has recently been included on the Red List of the International Union for Conservation of Nature (IUCN) as a critically endangered species. The rearing of Anguilla larvae is seen as a key bottleneck to the mass production of glass eels since very little ecological information is available regarding their natural nutrition. Studies of digestive physiology and ontogenetic development in eel larvae could provide useful information for solving some of the puzzles regarding larval fish culture. The aim of this study was to characterize the ontogeny of pancreatic enzymes (trypsin, lipase and amylase) and a peptide hormone regulator of pancreatic secretion (cholecystokinin) in terms of gene expression in European eel larvae from day 0 (P0) of hatching to 5, 10, 15 and 20 days post hatching during fasting. The results in the present study showed that all the genes selected were present, with different levels of expression and increasing trends, during larval development. At P0, the increase in the gene expression of lipase and amylase was higher than that of trypsin and cholecystokinin, confirming that enzymatic activity began before mouth opening and that larvae, provided with a complete enzymatic set, might have the capacity of digesting and absorbing various nutrients.

欧洲鳗鱼最近已被列为国际濒危物种红色名录。饲养鳗gu幼虫被视为大规模生产玻璃鳗的关键瓶颈，因为关于其天然营养的生态信息很少。鳗幼虫消化生理学和个体发育的研究可以为解决有关幼虫鱼类养殖的一些难题提供有用的信息。这项研究的目的是从孵化第0天（P0）至第5天欧洲鳗幼虫的基因表达来表征胰腺酶（胰蛋白酶，脂肪酶和淀粉酶）的个体发育以及胰腺分泌的肽激素调节剂（胆囊收缩素）。禁食后第10、15和20天。本研究的结果表明，在幼体发育过程中，所有选择的基因均以不同的表达水平和增加的趋势存在。在P0时，脂肪酶和淀粉酶的基因表达增加高于胰蛋白酶和胆囊收缩素，这表明酶活性在张口之前就已经开始，并且幼虫具有完整的酶集合，可能具有消化和吸收各种酶的能力。营养。