Obesity has been reported to induce oxidative stress, inflammation and apoptosis in the testis. The objective of this study was to determine the effects of the anti-obesity drug orlistat, on testicular oxidative stress, inflammation and apoptosis in high-fat diet (HFD)-fed rats. Twenty-four adult male Sprague Dawley rats weighing 250−300 g were randomized into four groups (n = 6/group), namely; normal control (NC), high-fat diet (HFD), HFD plus orlistat (10 mg/kg body weight/day administered concurrently for 12 weeks) (HFD + Opr) and HFD plus orlistat (10 mg/kg body weight/day administered 6 weeks after induction of obesity) (HFD + Ot) groups. Antioxidant enzymes activities were significantly decreased, while mRNA levels of pro-apoptotic markers (p53, Bax/BCl-2, caspase-9, caspase-8 and caspase-3) were significantly increased in the testis of HFD group relative to NC group. Furthermore, the mRNA levels of pro-inflammatory markers (nuclear factor kappa B, inducible nitric oxide synthase, tumor necrosis factor alpha and interleukin (IL)-1β increased significantly, while anti-inflammatory marker (IL-10) decreased significantly in the testis of the HFD group relative to NC group. However, in both models of orlistat intervention (protective and treatment models) up-regulated antioxidant enzymes, down-regulated inflammation and apoptosis were observed in the testis of HFD-fed rats. Orlistat ameliorated testicular dysfunction by attenuating oxidative stress, inflammation and apoptosis in HFD-fed rats, suggesting its potential protective and therapeutic effects in the testis compromised by obesity.

据报道，肥胖症会引起睾丸氧化应激，炎症和细胞凋亡。这项研究的目的是确定抗肥胖药奥利司他对高脂饮食（HFD）喂养的大鼠睾丸氧化应激，炎症和细胞凋亡的影响。将24只体重250-300 g的成年雄性Sprague Dawley大鼠随机分为四组（n = 6 /组），即；正常对照（NC），高脂饮食（HFD），HFD加奥利司他（10 mg / kg体重/天，同时给药12周）（HFD + Opr）和HFD加奥利司他（10 mg / kg体重/天（肥胖诱导（HFD + Ot））组6周后服用。与NC组相比，HFD组的睾丸中抗氧化酶活性显着下降，而促凋亡标记物（p53，Bax / BCl-2，caspase-9，caspase-8和caspase-3）的mRNA水平显着升高。此外，睾丸中促炎性标志物（核因子κB，诱导型一氧化氮合酶，肿瘤坏死因子α和白介素（IL）-1β）的mRNA水平显着升高，而抗炎性标志物（IL-10）在睾丸中显着下降。 HFD组相对于NC组的比例。在奥利司他干预的两种模型（保护和治疗模型）中，在喂食HFD的大鼠睾丸中均观察到抗氧化酶上调，炎症和细胞凋亡下调。奥利司他通过减轻高脂饮食喂养的大鼠的氧化应激，炎症和细胞凋亡而改善了睾丸功能障碍，表明其在肥胖中损害了睾丸的潜在保护和治疗作用。