In order to effectively avoid the side effects induced by multiple components and tedious synthesis process, a simple therapy system based on one material to simultaneously realize both photothermal therapy (PTT) and photodynamic therapy (PDT) under single laser irradiation will promote the overall phototherapeutic efficiency and make the PTT/PDT system easier to operate. Here, by using transferrin (Tf) as protein template, ultrasmall CuS@transferrin nanodots (CuS@Tf NDs) were successfully synthesized through a facile one-pot protein-based biomineralization method. The obtained CuS@Tf NDs exhibited not only excellent photothermal conversion ability (34.4%) but also high photoactivated formation of reactive oxygen species (ROS) upon 980 nm near-infrared (NIR) irradiation. By loading the drug doxorubicin (DOX) to CuS@Tf NDs, a synergistic therapy system with multiple therapeutic effects combined PTT, PDT, chemotherapy (CT) and tumor targeting properties could be perfectly implemented together by CuS@Tf-DOX NDs without any complicated post-modification process. Results from the in vitro cell experiments confirmed that these CuS@Tf-DOX NDs could produce excellent effect on cancer cells with 88.5% cell inhibition rate. In comparison with the complicated systems based on “multiple-components-in-one” strategy, this therapy system based on one single material but possess multifunctional purpose is easy to operate and more suitable for clinical applications.

为了有效避免由多种成分和繁琐的合成过程引起的副作用，基于一种材料的简单治疗系统在单激光照射下同时实现光热疗法（PTT）和光动力疗法（PDT），将提高总体光疗效率并使PTT / PDT系统更易于操作。在这里，以转铁蛋白（Tf）为蛋白质模板，通过一种简便的基于一锅蛋白质的生物矿化方法成功合成了超小CuS @转铁蛋白纳米点（CuS @ Tf NDs）。所获得的CuS @ Tf NDs不仅表现出优异的光热转化能力（34.4％），而且在980 nm近红外（NIR）辐照下还具有高光活化活性氧（ROS）的形成。通过将药物阿霉素（DOX）加载到CuS @ Tf ND，CuS @ Tf-DOX ND可以完美地实现具有PTT，PDT，化学疗法（CT）和肿瘤靶向特性相结合的多种治疗效果的协同治疗系统，而无需任何复杂的后修饰过程。体外细胞实验的结果证实，这些CuS @ Tf-DOX NDs可以以88.5％的细胞抑制率对癌细胞产生优异的作用。与基于“多组分合一”策略的复杂系统相比，这种基于单一材料但具有多功能目的的治疗系统易于操作，更适合于临床应用。体外细胞实验的结果证实，这些CuS @ Tf-DOX NDs可以以88.5％的细胞抑制率对癌细胞产生优异的作用。与基于“多组分合一”策略的复杂系统相比，这种基于单一材料但具有多功能目的的治疗系统易于操作，更适合于临床应用。体外细胞实验的结果证实，这些CuS @ Tf-DOX NDs可以以88.5％的细胞抑制率对癌细胞产生优异的作用。与基于“多组分合一”策略的复杂系统相比，这种基于单一材料但具有多功能目的的治疗系统易于操作，更适合于临床应用。