Abstract Background The effects of dietary nutrition on tail fat deposition and the correlation between production performance and the Hh signaling pathway and OXCT1 were investigated in fat-tailed sheep. Tan sheep were fed different nutritional diets and the variances in tail length, width, thickness and tail weight as well as the mRNA expression of fat-related genes (C/EBPα, FAS, LPL, and HSL) were determined in the tail fat of sheep at three different growth stages based on their body weight. Furthermore, the correlations between tail phenotypes and the Hedgehog (Hh) signaling pathway components (IHH, PTCH1, SMO, and GLI1) and OXCT1 were investigated. Results C/EBPα, FAS, LPL, and HSL were expressed with differences in tail fat of sheep fed different nutritional diets at three different growth stages. The results of the two-way ANOVA showed the significant effect of nutrition, stage, and interaction on gene expression, except the between C/EBPα and growth stage. C/EBPα, FAS, and LPL were considerably correlated with the tail phenotypes. Furthermore, the results of the correlation analysis demonstrated a close relationship between the tail phenotypes and Hh signaling pathway and OXCT1. Conclusions The present study demonstrated the gene-level role of dietary nutrition in promoting tail fat deposition and related tail fat-related genes. It provides a molecular basis by which nutritional balance and tail fat formation can be investigated and additional genes can be identified. The findings of the present study may help improve the production efficiency of fat-tailed sheep and identify crucial genes associated with tail fat deposition. How to cite: Zeng J, Zhou S, Yang Y, et al. Effect of dietary nutrition on tail fat deposition and evaluation of tail-related genes in fat-tailed sheep. Electron J Biotechnol 2020;46. https://doi.org/10.1016/j.ejbt.2020.05.004 .

中文翻译：

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日粮营养对肥尾羊尾部脂肪沉积的影响及尾部相关基因的评价

摘要 背景研究了日粮营养对肥尾羊尾部脂肪沉积的影响以及生产性能与Hh信号通路和OXCT1的相关性。对滩羊饲喂不同营养日粮，测定其尾肥中尾长、尾宽、尾厚、尾重的变化以及脂肪相关基因（C/EBPα、FAS、LPL、HSL）的mRNA表达量。羊根据体重分为三个不同的生长阶段。此外，研究了尾部表型与刺猬 (Hh) 信号通路组件（IHH、PTCH1、SMO 和 GLI1）和 OXCT1 之间的相关性。结果 C/EBPα、FAS、LPL 和 HSL 的表达随饲喂不同营养日粮的绵羊在三个不同生长阶段的尾部脂肪含量存在差异。双向方差分析的结果表明，除了 C/EBPα 和生长阶段之间，营养、阶段和相互作用对基因表达的影响显着。C/EBPα、FAS 和 LPL 与尾部表型显着相关。此外，相关性分析的结果表明尾部表型和 Hh 信号通路与 OXCT1 之间存在密切关系。结论 本研究证明了膳食营养在促进尾脂肪沉积和相关尾脂肪相关基因中的基因水平作用。它提供了一个分子基础，通过它可以研究营养平衡和尾部脂肪的形成，并可以识别其他基因。本研究的结果可能有助于提高肥尾羊的生产效率，并确定与尾脂肪沉积相关的关键基因。如何引用：曾杰，周生，杨 Y，等。日粮营养对肥尾羊尾脂肪沉积的影响及尾相关基因评价[J]. 电子 J 生物技术 2020；46。https://doi.org/10.1016/j.ejbt.2020.05.004。