The membraneless messenger ribonucleoprotein (mRNP) granules, including processing bodies (PBs) and stress granules (SGs), are important cytoplasmic structures in eukaryotes that can participate in gene expression through mRNA regulation. It has been verified that mRNP granules are mainly composed of proteins and translation-repressed mRNAs. Here, we reported a stop-codon read-through gene, At3g52980, in plants for the first time. At3g52980 encodes a novel non-tandem CCCH zinc-finger (non-TZF) protein named AtC3H18-Like (AtC3H18L), which contains two putative RNA-binding domains. By using transient expression system, we showed that heat treatment can induce the aggregation of diffuse distributed AtC3H18L to form cytoplasmic foci, which were similar to PBs and SGs in morphology. Further analysis did find that AtC3H18L can co-localize with markers of PB and SG. The aggregation of AtC3H18L was closely related to the cytoskeleton, and AtC3H18L-foci were highly dynamic and can move frequently along cytoskeleton. Moreover, analysis in transgenic plants showed that AtC3H18L was specifically expressed in pollen and can form cytoplasmic foci without heat treatment. It will be fascinating in future studies to discover whether and how AtC3H18L affects pollen development by participating in the assembly of mRNP granules as a protein component, especially under heat stress.

无膜信使核糖核蛋白（mRNP）颗粒，包括加工体（PB）和应激颗粒（SG），是真核生物中重要的细胞质结构，可以通过mRNA调控参与基因表达。已证实mRNP颗粒主要由蛋白质和翻译抑制的mRNA组成。在这里，我们首次报道了植物中的终止密码子通读基因At3g52980。 At3g52980 编码一种名为 AtC3H18-Like (AtC3H18L) 的新型非串联 CCCH 锌指（非 TZF）蛋白，其包含两个假定的 RNA 结合域。通过使用瞬时表达系统，我们发现热处理可以诱导弥漫分布的AtC3H18L聚集形成细胞质焦点，其形态与PB和SG相似。进一步分析确实发现AtC3H18L可以与PB和SG标记物共定位。 AtC3H18L的聚集与细胞骨架密切相关，AtC3H18L-foci具有高度动态性，可以沿着细胞骨架频繁移动。此外，对转基因植物的分析表明，AtC3H18L在花粉中特异性表达，并且无需热处理即可形成细胞质灶。在未来的研究中，发现 AtC3H18L 是否以及如何通过参与 mRNP 颗粒作为蛋白质成分的组装（尤其是在热应激下）来影响花粉发育将是令人着迷的。