This study aims to evaluate the effect of a new type of collagen crosslinking (CXL) mediated by microbial transglutaminases (Tgases) on sclera. Porcine eyes were divided into two groups according to the different crosslinking procedures used: the double-sided CXL group (D-CXL group) and the single-sided CXL group (S-CXL group). In the D-CXL group, 4.0 × 14.0 mm scleral strips harvested from 40 porcine eyeballs were incubated with 1 U/ml Tgases for 30 min at 37 °C. Parallel scleral strips from the same eyeball were incubated with PBS under the same conditions as the controls. In the S-CXL group, 80 whole globes were directly incubated with 1 U/ml Tgases and PBS as the controls for 30 min at 37 °C. After incubation, 4.0 × 14.0 mm scleral strips were cut from each eyeball. Biomechanical testing and light microscopy were used. In the D-CXL group, the general elastic modulus of the Tgases-treated scleral strips was 14.89 ± 6.05 MPa, and the controls was 6.72 ± 2.58 MPa, indicating an increase of 121% with Tgases treatment. In the S-CXL group, the general elastic modulus of the Tgases-treated scleral strips was 12.88 ± 4.29 MPa, and the controls was 7.00 ± 2.45 MPa, indicating an increase of 84% with Tgases treatment. In both the D-CXL and S-CXL groups, significant increases in scleral rigidity were observed compared to that of the respective controls (P < 0.05). The histology indicated increased collagen bundle density, decreased interfibrillar spaces and increased interlamellar spaces after CXL. In conclusion, scleral collagen crosslinking mediated by Tgases produced a significant increase in biomechanical strength.

这项研究旨在评估微生物转谷氨酰胺酶（Tgas）介导的新型胶原交联（CXL）对巩膜的影响。根据所使用的不同交联程序，将猪眼分为两组：双面CXL组（D-CXL组）和单面CXL组（S-CXL组）。在D-CXL组中，将从40个猪眼球收集的4.0×14.0 mm巩膜条与1 U / ml Tgas在37°C下孵育30分钟。在与对照相同的条件下，将同一眼球的平行巩膜条与PBS孵育。在S-CXL组中，将80个全球直接与1 U / ml Tgas和PBS作为对照在37°C下孵育30分钟。孵育后，从每个眼球切下4.0×14.0 mm巩膜条。使用了生物力学测试和光学显微镜。在D-CXL组中，经Tgases处理的巩膜条的总弹性模量为14.89±6.05 MPa，而对照组为6.72±2.58 MPa，表明经Tgases处理后增加了121％。在S-CXL组中，经Tgases处理的巩膜条的总体弹性模量为12.88±4.29 MPa，而对照组为7.00±2.45 MPa，表明经Tgases处理后，弹性模量增加了84％。在D-CXL和S-CXL组中，与相应对照组相比，巩膜刚度均显着增加（P <0.05）。组织学表明CXL后胶原束密度增加，原纤维间空间减少和层间空间增加。总之，由Tgas介导的巩膜胶原交联作用显着提高了生物力学强度。89±6.05 MPa，而对照组为6.72±2.58 MPa，表明Tgases处理提高了121％。在S-CXL组中，经Tgases处理的巩膜条的总弹性模量为12.88±4.29 MPa，而对照组为7.00±2.45 MPa，表明经Tgases处理后，其弹性模量增加了84％。在D-CXL和S-CXL组中，与相应对照组相比，巩膜刚度均显着增加（P <0.05）。组织学表明CXL后胶原束密度增加，原纤维间空间减少和层间空间增加。总之，由Tgas介导的巩膜胶原交联作用显着提高了生物力学强度。89±6.05 MPa，而对照组为6.72±2.58 MPa，表明Tgases处理提高了121％。在S-CXL组中，经Tgases处理的巩膜条的总体弹性模量为12.88±4.29 MPa，而对照组为7.00±2.45 MPa，表明经Tgases处理后，弹性模量增加了84％。在D-CXL和S-CXL组中，与相应对照组相比，巩膜刚度均显着增加（P <0.05）。组织学表明CXL后胶原束密度增加，原纤维间空间减少和层间空间增加。总之，由Tgas介导的巩膜胶原交联作用显着提高了生物力学强度。经Tgases处理的巩膜条的总弹性模量为12.88±4.29 MPa，而对照组为7.00±2.45 MPa，表明经Tgases处理后增加了84％。在D-CXL和S-CXL组中，与相应对照组相比，巩膜刚度均显着增加（P <0.05）。组织学表明，CXL后胶原束密度增加，原纤维间空间减少，层间空间增加。总之，由Tgas介导的巩膜胶原交联作用显着提高了生物力学强度。经Tgases处理的巩膜条的总弹性模量为12.88±4.29 MPa，而对照组为7.00±2.45 MPa，表明经Tgases处理后增加了84％。在D-CXL和S-CXL组中，与相应对照组相比，巩膜刚度均显着增加（P <0.05）。组织学表明CXL后胶原束密度增加，原纤维间空间减少和层间空间增加。总之，由Tgas介导的巩膜胶原交联作用显着提高了生物力学强度。与各个对照组相比，观察到巩膜刚度显着增加（P <0.05）。组织学表明CXL后胶原束密度增加，原纤维间空间减少和层间空间增加。总之，由Tgas介导的巩膜胶原交联作用显着提高了生物力学强度。与各个对照组相比，观察到巩膜刚度显着增加（P <0.05）。组织学表明CXL后胶原束密度增加，原纤维间空间减少和层间空间增加。总之，由Tgas介导的巩膜胶原交联作用显着提高了生物力学强度。