This study investigated the influence of probiotic Pediococcus acidilactici MA 18/5 M on the metabolic enzyme activities and mRNA levels of growth, immunity, and antioxidative-related genes in zebrafish. Zebrafish fed with diets containing P. acidilactici MA 18/5 M at 0, 10⁷, 10⁸, and 10⁹ CFU/g levels for eight weeks. The obtained results revealed that the creatine phosphokinase, lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase remained unchanged in all groups (P > 0.05). Dietary P. acidilactici enhanced protease activity relative to the control group (P < 0.05). Compared with the non-treated group, lysozyme activity increased in fish fed with high and medium doses of P. acidilactici, but the mRNA level of lysozyme gene was only up-regulated in the fish received the high dose of P. acidilactici (P < 0.05). Meanwhile, gene expressions of IGF-1, TNF-α, GH-1, and catalase were increased by P. acidilactici at 10⁷ CFU/g relative to the control group. Conversely, the expression of glutathione S-reductase (GSR) decreased in zebrafish fed with medium and high doses of P. acidilactici compared to the control group. Thus, dietary administration of P. acidilactici MA 18/5 M, especially at 10⁷ CFU/g diet, could improve the growth, immunity, and health status of zebrafish.

这项研究调查了益生菌乳酸双球菌MA 18/5 M对斑马鱼代谢酶活性以及生长，免疫和抗氧化相关基因的mRNA水平的影响。斑马鱼饲喂含饮食乳酸片球菌MA 18/5 M达0，10 ⁷，10 ⁸，10 ⁹八周CFU / g的水平。获得的结果表明，所有组中的肌酸磷酸激酶，乳酸脱氢酶，碱性磷酸酶，丙氨酸氨基转移酶和天冬氨酸氨基转移酶均保持不变（P  > 0.05）。饮食乳酸乳酸菌相对于对照组（P <0.05）。与未治疗组相比，饲喂高剂量和中等剂量嗜酸乳酸杆菌的鱼的溶菌酶活性增加，但溶菌酶基因的mRNA水平仅在接受高剂量嗜酸乳酸杆菌的鱼中被上调（P  < 0.05）。同时，乳酸假单胞菌以10 ⁷ CFU / g的浓度较对照组增加了IGF-1，TNF-α，GH-1和过氧化氢酶的基因表达。相反，与中，高剂量的嗜酸乳酸杆菌相比，斑马鱼中谷胱甘肽S-还原酶（GSR）的表达下降。因此，饮食中嗜酸乳酸杆菌MA 18/5 M，尤其是在10 ^7时 CFU / g饮食可以改善斑马鱼的生长，免疫力和健康状况。