Green microalgae are able to sustain hydrogen evolution in the light under nutrient deprivation conditions. In the current study, the role of hydrogen photoproduction in the acclimation of Chlamydomonas reinhardtii cells to nutrient deficiency was investigated. In this regard, such physiological characteristics of algal cells as starch and adenosine triphosphate content, fatty acid composition, fermentation products, photosynthetic activity, cell mortality, culture recovery, and cell ultrastructure were compared between the hydEF-1 mutant lacking hydrogenase activity and its parent strain CC-425 under sulfur deficiency. The results showed an increased number of non-viable cells, altered cell ultrastructure, and modified lipid, carbohydrate, and energy metabolism in the mutant cells. We suggest that active hydrogenase provides benefits for algae acclimation to the anaerobic phase of nutrient deprivation by regulating intracellular redox and pH balance and improving performance of anaerobic metabolism.

在营养缺乏的条件下，绿色微藻能够在光照下维持氢的释放。在当前的研究中，研究了氢光生产在莱茵衣藻对营养缺乏的适应中的作用。在这方面，比较了hydEF-1等藻类细胞的生理特性，如淀粉和三磷酸腺苷含量，脂肪酸组成，发酵产物，光合活性，细胞死亡率，培养物回收率和细胞超微结构。缺乏氢化酶活性的突变体及其在硫缺乏下的亲本CC-425。结果表明，突变细胞中无活力细胞数量增加，细胞超微结构改变，脂质，碳水化合物和能量代谢发生改变。我们建议活性氢酶通过调节细胞内的氧化还原和pH平衡并改善厌氧代谢的性能，为藻类适应营养缺乏的厌氧期提供有益的条件。