Cinnamomum bodinieri is a useful plant that could be improved by increasing its cultivation rate. To explore the optimal concentration of exogenously applied IBA for root formation and development in the C. bodinieri citral type, micro-shoots and the fresh tissues of their bases were subjected to morphological and transcriptome analyses, respectively. When plantedQuery on a rooting medium (1/2 MS) with 1.5 mg/L IBA for 25 days, the rooting rates and root numbers were significantly higher than those obtained with 0, 0.5, 1.0, or 2.0 mg/L IBA treatment. Transcriptome analyses conducted 0 day, 7 days, 11 days, and 14 days after planting in the optimal rooting medium demonstrated that the IBA modified the plant’s carbohydrate metabolism and auxin signaling pathway. The root-specific expressions of TIR1, GH3, and SAUR were up-expressed while AUX/IAA was repressed, which contributed to the formation of lateral root initiation sites and root cell elongation. At the same time, d-glucose synthesis increased and the accumulation of starch decreased as more carbohydrates were used to form and develop roots instead of being used for the rapid cell division required for shoot growth. This study identified the optimal rooting medium for the C. bodinieri citral type and determined the molecular mechanisms involved in the regulation of exogenously applied IBA during root formation and development at the transcriptional level.

中文翻译：

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转录组学分析IBA对肉桂木柠檬型生根能力的影响

Cinnamomum bodinieri是一种有用的植物，可以通过提高其栽培率来进行改良。为了探索外生IBA在柠檬酸橙中的根形成和发育的最佳浓度，分别对微芽和其基部的新鲜组织进行了形态学和转录组分析。将查询种植在具有1.5 mg / L IBA的生根培养基（1/2 MS）上25天时，生根率和根数显着高于0、0.5、1.0或2.0 mg / L IBA处理获得的生根率和根数。在最佳生根培养基中种植后0天，7天，11天和14天进行的转录组分析表明，IBA修饰了植物的碳水化合物代谢和植物生长素信号传导途径。的根特定表达式TIR1，GH3和SAUR被上调，而AUX / IAA被下调，这有助于侧根起始位点的形成和根细胞伸长。同时，随着更多的碳水化合物用于形成和发展根而不是用于芽生长所需的快速细胞分裂，d-葡萄糖的合成增加，淀粉的积累减少。这项研究确定了C. bodinieri柠檬醛型的最佳生根培养基，并确定了在转录水平上根形成和发育过程中调控外源施用IBA的分子机制。