Accumulating evidence supports that Sirtuin 6 (SIRT6) may play a vital role in the pathogenesis of spinal cord injury. The current study was designed to investigate the specific effects of SIRT6 on spinal cord injury (SCI). HE and Nissl staining were performed for pathological analysis in SCI rats. SIRT6 expression was detected by RT-qPCR. CCK8 assay was applied for the detection of cell viability of LPS-injured PC12 cells. TNF-a, IL-1β, IL-6, MCP-1 levels and ROS, MPO, SOD levels were assessed to evaluate inflammation and oxidative stress in spinal cord injury. Cell apoptosis were evaluated by morphological examination using AO/EB fluorescent staining methods and key proteins related to apoptosis were explored via western blot. HE staining revealed increased cavity involving the dorsal white matter and central gray matter, and Nissl staining discovered the loss of motor neurons in the ventral horn in SCI rats. SIRT6 had lower expression in SCI rats. Lipopolysaccharide (LPS) exposure induced cell apoptosis and reduced the expression of SIRT6. Mechanistically, we revealed that up-regulation of SIRT6 alleviated inflammation and oxidative stress and inhibited cell apoptosis in spinal cord injury. Together, our findings indicated that SIRT6 attenuated spinal cord injury by suppressing inflammation, oxidative stress, and cell apoptosis. This study demonstrates that SIRT6 may represent a protective effect against spinal cord injury.

中文翻译：

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SIRT6对脊髓损伤中的炎症，氧化应激和细胞凋亡的保护作用。

越来越多的证据支持Sirtuin 6（SIRT6）可能在脊髓损伤的发病机理中起着至关重要的作用。本研究旨在研究SIRT6对脊髓损伤（SCI）的特定作用。在SCI大鼠中进行HE和Nissl染色以进行病理分析。通过RT-qPCR检测SIRT6表达。CCK8检测用于检测LPS损伤的PC12细胞的细胞活力。评估TNF-α，IL-1β，IL-6，MCP-1水平和ROS，MPO，SOD水平，以评估脊髓损伤中的炎症和氧化应激。使用AO / EB荧光染色方法通过形态学检查评估细胞凋亡，并通过蛋白质印迹探索与凋亡相关的关键蛋白。HE染色发现腔内累及背白质和中央灰质，Nissl染色发现SCI大鼠腹角运动神经元丢失。SIRT6在SCI大鼠中的表达较低。脂多糖（LPS）暴露诱导细胞凋亡并降低SIRT6的表达。从机制上讲，我们发现SIRT6的上调减轻了炎症和氧化应激并抑制了脊髓损伤中的细胞凋亡。总之，我们的发现表明SIRT6通过抑制炎症，氧化应激和细胞凋亡来减轻脊髓损伤。这项研究表明SIRT6可能代表对脊髓损伤的保护作用。我们发现SIRT6的上调减轻了炎症和氧化应激并抑制了脊髓损伤中的细胞凋亡。总之，我们的发现表明SIRT6通过抑制炎症，氧化应激和细胞凋亡来减轻脊髓损伤。这项研究表明SIRT6可能代表对脊髓损伤的保护作用。我们发现SIRT6的上调减轻了炎症和氧化应激并抑制了脊髓损伤中的细胞凋亡。总之，我们的发现表明SIRT6通过抑制炎症，氧化应激和细胞凋亡来减轻脊髓损伤。这项研究表明SIRT6可能代表对脊髓损伤的保护作用。