Trichloroethylene (TCE) is the most ubiquitous halogenated organic pollutant in the environment, it is one of the 129 priority control pollutants. In order to clarify the influence of TCE on microorganisms and nitrogen transformation in Mollisol is the core purpose of this study. Results showed that 10 mg kg-1 TCE is the concentration limit of ammonification in Mollisol. When the concentration of TCE reached 10 mg kg-1 and the effect lasted for over 7 days, the process of ammonia oxidation to nitric acid in Mollisol will be affected. TCE affected the process of nitrate (NO3-) transformation into nitrite (NO2-) by affecting the activity of nitrate reductase, thereby affected the denitrification process in soil. When the concentration of TCE is more than 10 mg kg-1 it reduced the ability of soil microorganisms to obtain nitrogen, thereby affecting soil nitrogen transformation. RDA (Redundancy analysis) showed that the activity of nitrate reductase and the number of nitrifying bacteria and denitrifying bacteria in soil was negatively correlated with the incubation of TCE. In addition, soil nitrate reductase, nitrite reductase, peroxidase activity, ammonifying bacteria, nitrifying bacteria and denitrifying bacteria were negatively correlated with TCE concentration. Beyond that PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) of functional gene structure depend on KEGG (Kyoto Encyclopedia of Genes and Genomes) showed that 20 mg kg-1 TCE significantly inhibited the metabolism of energy and other substances in Mollisol. Based on the above, it is found that TCE significantly affected nitrification and denitrification in Mollisol, thus the nitrogen transformation in Mollisol was affected by TCE contamination.

中文翻译：

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三氯乙烯抑制了Mollisol中的氮转化和微生物群落结构。

三氯乙烯（TCE）是环境中最普遍存在的卤化有机污染物，它是129种优先控制的污染物之一。为了阐明TCE对微生物的影响以及在Mollisol中氮的转化是本研究的核心目的。结果表明，10 mg kg-1 TCE是氨水中氨化的浓度极限。当TCE的浓度达到10 mg kg-1并持续7天以上时，会影响氨水在Mollisol中氧化为硝酸的过程。TCE通过影响硝酸盐还原酶的活性来影响硝酸盐（NO3-）转化为亚硝酸盐（NO2-）的过程，从而影响土壤的反硝化过程。当TCE的浓度超过10 mg kg-1时，它会降低土壤微生物获得氮的能力，从而影响土壤氮素转化。RDA（冗余分析）表明，土壤中硝酸还原酶的活性以及硝化细菌和反硝化细菌的数量与TCE的培养呈负相关。此外，土壤硝酸盐还原酶，亚硝酸盐还原酶，过氧化物酶活性，氨化细菌，硝化细菌和反硝化细菌与三氯乙烯浓度呈负相关。除此之外，功能基因结构的PICRUSt（通过未观察到的状态重建社区的系统发育研究）取决于KEGG（基因和基因组京都百科全书）表明，20 mg kg-1 TCE能够显着抑制Mollisol中能量和其他物质的代谢。基于上述发现，TCE显着影响了Mollisol中的硝化和反硝化作用，