The occurrence of multidrug-resistant pathogenic bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Acinetobacter baumannii (MDRAB), extended-spectrum β-lactamase (ESBL) Escherichia coli, and Pseudomonas aeruginosa, has become a serious problem in animals and public. The objective of this study was to identify and isolate lactic acid bacterial (LAB) strains from the intestinal tracts of pigs and feces of dogs and then characterize them as potential probiotics with antimicrobial activity against multidrug-resistant pathogenic bacteria. In a preliminary isolation screening, 45 of 1167 isolated LAB strains were found to have anti-S. aureus ATCC 27,735 activity. Using 16S rDNA and 16S-23S rDNA intergenic spacer region (ISR) sequences, five of these isolates were further identified as Lactobacillus animalis 30a-2, Lactobacillus reuteri 4-12E, Weissella cibaria C34, Lactococcus lactis 5-12H, and Lactococcus lactis 6-3H. Antimicrobial substance assays suggest that the L. lactis 5-12H, L. lactis 6-3H, L. animalis 30a-2, L. reuteri 4-12E, and W. cibaria C34 strains might produce bacteriocins and hydrogen peroxide (H2O2) as antimicrobial substances. The L. animalis 30a-2 and W. cibaria C34 strains were further characterized for probiotic properties and shown to have high acid and bile salt tolerance. Additionally, they have broad antimicrobial spectra, and can significantly repress the growth of all of the tested strains of MRSA isolates, some MDRAB, ESBL E. coli, and P. aeruginosa isolates, along with food-borne pathogenic bacteria such as Bacillus cereus ATCC 11778, Listeria monocytogens ATCC 19111, Salmonella spp., Shigella spp., and Yersinia enterocolitica BCRC 12986. This is the first report of H2O2-producing L. animalis 30a-2 and W. cibaria C34 isolated from the intestinal tracts of pigs and feces of dogs that have good antimicrobial activity against multidrug-resistant and food-borne pathogenic bacteria and have excellent probiotic properties.

中文翻译：

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从猪肠道和狗粪便中分离的乳杆菌菌株的潜在益生菌对多重耐药病原菌具有抗菌活性

耐甲氧西林金黄色葡萄球菌（MRSA）、耐多药鲍曼不动杆菌（MDRAB）、超广谱β-内酰胺酶（ESBL）大肠杆菌、铜绿假单胞菌等多重耐药病原菌的出现成为严重问题在动物和公众中。本研究的目的是从猪的肠道和狗的粪便中鉴定和分离乳酸菌 (LAB) 菌株，然后将它们表征为对多重耐药病原菌具有抗菌活性的潜在益生菌。在初步分离筛选中，发现 1167 个分离的 LAB 菌株中有 45 个具有抗 S。金黄色葡萄球菌 ATCC 27,735 活动。使用 16S rDNA 和 16S-23S rDNA 基因间隔区 (ISR) 序列，其中五个分离株被进一步鉴定为动物乳杆菌 30a-2，罗伊氏乳杆菌 4-12E、魏氏乳杆菌 C34、乳酸乳球菌 5-12H 和乳酸乳球菌 6-3H。抗菌物质测定表明，乳酸乳球菌 5-12H、乳酸乳球菌 6-3H、动物乳球菌 30a-2、罗伊氏乳杆菌 4-12E 和 W.cibaria C34 菌株可能产生细菌素和过氧化氢 (H2O2)，如抗菌物质。L.animalis 30a-2 和 W. cibaria C34 菌株进一步表征了益生菌特性，并显示出具有高酸和胆盐耐受性。此外，它们具有广泛的抗菌谱，并且可以显着抑制所有 MRSA 分离株、一些 MDRAB、ESBL 大肠杆菌和铜绿假单胞菌分离株以及食源性致病菌（如蜡状芽孢杆菌 ATCC）的生长11778，单核细胞增生李斯特菌 ATCC 19111，沙门氏菌属，志贺氏菌属，