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Shimazuella alba sp. nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007
Archives of Microbiology ( IF 2.3 ) Pub Date : 2020-05-23 , DOI: 10.1007/s00203-020-01894-z
Hayrettin Saygin 1, 2 , Hilal Ay 2 , Nevzat Sahin 2
Affiliation  

A novel, Gram-stain-positive bacterium, designated KC615T, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Whole-cell hydrolysates contained ribose and glucose. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and hydroxy-phosphatidylethanolamine. The predominant menaquinones (> 10%) were MK-9(H4) and MK-10(H4). Major fatty acids were anteiso-C15:0, C20:0 and C18:0. The genomic DNA G + C content observed for strain KC615T was 38.5 mol%. Based on 16S rRNA gene similarity, DNA–DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp. nov. is proposed. The type strain is KC615T (= JCM 33532T = CGMCC 4.7616T).

中文翻译:

Shimazuella alba sp. 十一月 从沙漠土壤中分离出来并修正了对 Shimazuella Park 等人的描述。2007年

从土库曼斯坦卡拉库姆沙漠采集的沙漠土壤中分离出一种新型革兰氏染色阳性细菌,命名为 KC615T。基于几乎完整的 16S rRNA 基因序列的系统发育分析表明,分离株 KC615T 与 Shimazuella kribbensis KCTC 9933T 形成了一个单系进化枝,具有 98.2% 的相似性,多相分类学研究证实了该菌株与 Shimazuella 属的隶属关系。细胞壁肽聚糖含有内消旋二氨基庚二酸。全细胞水解物含有核糖和葡萄糖。主要的极性脂质是二磷脂酰甘油、磷脂酰乙醇胺和羟基磷脂酰乙醇胺。主要的甲基萘醌 (> 10%) 是 MK-9(H4) 和 MK-10(H4)。主要脂肪酸为反异 C15:0、C20:0 和 C18:0。对菌株 KC615T 观察到的基因组 DNA G + C 含量为 38.5 mol%。基于 16S rRNA 基因相似性、DNA-DNA 杂交值、化学分类学特征和差异生理特性,菌株 KC615T 被认为代表 Shimazuella 属内的一个新物种,其名称 Shimazuella alba sp。十一月 被提议。类型菌株是 KC615T (= JCM 33532T = CGMCC 4.7616T)。
更新日期:2020-05-23
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