The in vitro cultures of Bacopa monnieri show poor production of the anti-Alzheimer’s drug, bacoside A. Therefore, suitable bioprocess optimization strategy was developed for callus induction from leaf explants (30 days), followed by callus proliferation (15 days). Central Composite Design was implemented to analyze the effect of pH, photoperiod, naphthalene acetic acid (NAA), and benzylaminopurine (BAP) concentration for maximum biosynthesis of bacoside A using leaf explants as well as callus explants as the inoculum. Using the CCD responses, it was predicted that the best biomass concentration of 4.56 ± 0.53 g/l DW and bacoside A production of 14.04 ± 1.31 mg/g DW can be obtained using 5.4 pH, 18 h/6 h L/D photoperiod regime, and 1.2 mg/l BAP in combination with 0.2 mg/l NAA. The kinetic parameter values for maximum specific growth rate (0.16/day), saturation constant (7.35 g/l), inhibition constant (120 g/l), biomass yield (0.011 g/g), maintenance coefficient (0.02 g/g/day), and growth-associated (0.627 mg/g) and non-growth-associated (1.096 mg/g/day) bacoside A formation constants were determined experimentally in batch cultures using optimized conditions.

巴氏杆菌的体外培养由于显示出抗阿尔茨海默氏病的药物bacoside A的生产不佳，因此，开发了合适​​的生物工艺优化策略，用于从叶外植体诱导愈伤组织（30天），然后使愈伤组织增殖（15天）。进行了中央复合设计以分析pH，光周期，萘乙酸（NAA）和苄基氨基嘌呤（BAP）浓度对使用叶子外植体和愈伤组织外植体作为接种物最大程度合成Bacoside A的影响。使用CCD响应，可以预测，在5.4 pH，18 h / 6 h L / D光周期条件下，最佳生物量浓度为4.56±0.53 g / l DW和bacoside A的产量为14.04±1.31 mg / g DW。 ，1.2 mg / l BAP和0.2 mg / l NAA的组合。最大比生长速率（0.16 /天），饱和常数（7.35 g / l），