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Hydrophobically modified carbon dots as a multifunctional platform for serum-resistant gene delivery and cell imaging.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-05-22 , DOI: 10.1039/d0bm00651c Ping Chen 1 , Ji Zhang , Xi He , Yan-Hong Liu , Xiao-Qi Yu
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-05-22 , DOI: 10.1039/d0bm00651c Ping Chen 1 , Ji Zhang , Xi He , Yan-Hong Liu , Xiao-Qi Yu
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The development of novel multifunctional gene delivery systems with high efficiency is significant. Herein, due to the unique physical and optical properties of carbon dots (CDs), CDs prepared from polyethyleneimine (PEI) were modified with various hydrophobic chains and different degrees of substitution via an epoxide ring-opening reaction. The modification and substitution degree were confirmed using several analytical methods including 1H NMR spectroscopy, FT-IR spectroscopy, TEM, and XPS. These CDs were utilized as multifunctional, safe and efficient non-viral gene vectors. The results showed that these materials possessed capability for dual-channel imaging, which enabled the intracellular tracking of the delivered DNA. Both the type and substitution degree of the hydrophobic chain have a large influence on their transfection efficiency. Among the prepared CDs, Ole1.5-CD gave the highest transfection efficiency, which was up to 200 times higher than that of PEI 25 kDa in the presence of serum in A549 cells. Meanwhile, these CD materials showed much less cytotoxicity and better serum tolerance than the traditional cationic polymeric gene vector. The cellular uptake assay further confirmed the good serum tolerance and structure–activity relationship of the CD materials. Thus, these CDs with good biocompatibility, self-imaging and high gene transfection efficiency may serve as a promising platform for both gene delivery and bio-imaging.
中文翻译：
疏水修饰的碳点可作为抗血清基因递送和细胞成像的多功能平台。
高效的新型多功能基因递送系统的开发意义重大。在本文中，由于碳点（CD）的独特物理和光学性质，由聚乙烯亚胺（PEI）制备的CD通过环氧化物开环反应被各种疏水链和不同取代度修饰。使用几种分析方法确定了修饰和取代度，包括11 H NMR光谱，FT-IR光谱，TEM和XPS。这些CD被用作多功能，安全和有效的非病毒基因载体。结果表明，这些材料具有双通道成像的能力，从而能够对递送的DNA进行细胞内跟踪。疏水链的类型和取代度都对其转染效率有很大影响。在准备的CD中，Ole1.5-CD具有最高的转染效率，在A549细胞中存在血清的情况下，其转染效率比PEI 25 kDa高200倍。同时，与传统的阳离子聚合基因载体相比，这些CD材料显示出更少的细胞毒性和更好的血清耐受性。细胞吸收测定进一步证实了CD材料具有良好的血清耐受性和构效关系。因此，这些具有良好生物相容性，自我成像和高基因转染效率的CD可以作为基因传递和生物成像的有前途的平台。
更新日期：2020-06-30
中文翻译：
疏水修饰的碳点可作为抗血清基因递送和细胞成像的多功能平台。
高效的新型多功能基因递送系统的开发意义重大。在本文中，由于碳点（CD）的独特物理和光学性质，由聚乙烯亚胺（PEI）制备的CD通过环氧化物开环反应被各种疏水链和不同取代度修饰。使用几种分析方法确定了修饰和取代度，包括11 H NMR光谱，FT-IR光谱，TEM和XPS。这些CD被用作多功能，安全和有效的非病毒基因载体。结果表明，这些材料具有双通道成像的能力，从而能够对递送的DNA进行细胞内跟踪。疏水链的类型和取代度都对其转染效率有很大影响。在准备的CD中，Ole1.5-CD具有最高的转染效率，在A549细胞中存在血清的情况下，其转染效率比PEI 25 kDa高200倍。同时，与传统的阳离子聚合基因载体相比，这些CD材料显示出更少的细胞毒性和更好的血清耐受性。细胞吸收测定进一步证实了CD材料具有良好的血清耐受性和构效关系。因此，这些具有良好生物相容性，自我成像和高基因转染效率的CD可以作为基因传递和生物成像的有前途的平台。
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