Continuous directed evolution methods allow the key steps of evolution-gene diversification, selection, and replication-to proceed in the laboratory with minimal researcher intervention. As a result, continuous evolution can find solutions much more quickly than traditional discrete evolution methods. Continuous evolution also enables the exploration of longer and more numerous evolutionary trajectories, increasing the likelihood of accessing solutions that require many steps through sequence space and greatly facilitating the iterative refinement of selection conditions and targeted mutagenesis strategies. Here we review the historical advances that have expanded continuous evolution from its earliest days as an experimental curiosity to its present state as a powerful and surprisingly general strategy for generating tailor-made biomolecules, and discuss more recent improvements with an eye to the future.

中文翻译：

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持续定向进化的开发工具包。

连续定向进化方法使进化基因多样化，选择和复制的关键步骤可以在实验室中以最少的研究人员干预进行。结果，连续进化可以比传统的离散进化方法更快地找到解决方案。连续进化还使得能够探索更长和更多的进化轨迹，增加了通过序列空间访问需要许多步骤的解决方案的可能性，并极大地促进了选择条件和目标诱变策略的迭代优化。在这里，我们将回顾从不断发展的连续性发展的历史进展，从最初的实验好奇心发展到现在的状态，将其作为一种强大且令人惊讶的通用策略来生成量身定制的生物分子，