Vascular Endothelial Growth Factor (VEGF) promotes angiogenesis in tumours of various cancers. Monoclonal antibodies and nanobodies are one of the potent agents in the treatment of cancer. Due to their high costs, researchers are considering to design and produce peptides as a substitute approach in recent years. The aim of the current study was designing a mimotope against VEGF and evaluate its effects on cell proliferation and tube formation in the HUVEC cell line. For this, a peptide was designed against VEGF and chemically produced. The effects of synthetic peptide and nanobody on the inhibition of proliferation of HUVEC cells were examined using MTT and tube formation assays. The data indicate that the peptide was able to significantly inhibit both HUVEC cell proliferation and tube formation through inhibition of VEGF, highlighting the potential of peptides as a 'novel' class of candidate drugs to inhibit angiogenesis.

中文翻译：

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纳米抗体针对VEGF的模拟表位抑制癌症血管生成。

血管内皮生长因子（VEGF）促进各种癌症的肿瘤中的血管生成。单克隆抗体和纳米抗体是治疗癌症的有效药物之一。由于成本高昂，研究人员近年来正在考虑设计和生产肽作为替代方法。本研究的目的是设计一种针对VEGF的模拟表位，并评估其对HUVEC细胞系中细胞增殖和管形成的影响。为此，设计了针对VEGF的肽并化学生产。使用MTT和管形成试验检查了合成肽和纳米抗体对HUVEC细胞增殖的抑制作用。数据表明，该肽能够通过抑制VEGF来显着抑制HUVEC细胞增殖和管形成，