A class of drugs called coxibs (COX-2 inhibitors) were created to help relieve pain and inflammation of osteoarthritis and rheumatoid arthritis with the lowest amount of side effects possible. The presented paper describes a new developed, optimized and validated thin layer chromatographic (TLC)-densitometric procedure for the simultaneous assay of five coxibs: celecoxib, etoricoxib, firecoxib, rofecoxib and cimicoxib. Chromatographic separation was conducted on HPTLC F₂₅₄ silica gel chromatographic plates as a stationary phase using chloroform–acetone–toluene (12:5:2, v/v/v) as a mobile phase. Densitometric detection was carried out at two wavelengths of 254 and 290 nm. The method was tested according to ICH guidelines for linearity, recovery and specificity. The presented method was linear in a wide range of concentrations for all analyzed compounds, with correlation coefficients greater than 0.99. The method is specific, precise (%RSD < 1) and accurate (more than 95%, %RSD < 2). Low-cost, simple and rapid, it can be used in laboratories for drug monitoring and quality control.

中文翻译：

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薄层色谱-光度法测定药物制剂中的五种考昔布

创建了一种名为coxibs（COX-2抑制剂）的药物，可帮助减轻骨关节炎和类风湿性关节炎的疼痛和炎症，并使副作用降至最低。本文介绍了一种新开发，优化和验证的薄层色谱（TLC）密度测定法，用于同时测定五种coxib：塞来昔布，依托昔布，firecoxib，rofecoxib和西米考昔。色谱分离在HPTLC F ₂₅₄硅胶色谱板上作为固定相进行，使用氯仿-丙酮-甲苯（12：5：2，v / v / v）作为流动相。在254和290nm的两个波长下进行光密度检测。根据ICH指南对线性，回收率和特异性进行了测试。对于所有分析的化合物，所提出的方法在宽浓度范围内都是线性的，相关系数大于0.99。该方法特异，精确（％RSD <1）和准确（大于95％，％RSD <2）。低成本，简单，快速，可在实验室中用于药物监测和质量控制。