An efficient micropropagation protocol for Rheum rhabarbarum L. was developed in this study. The in vitro rhubarb plants obtained in the multiplication stage (proliferation rate: 5.0 ± 0.5) were rooted in vitro (96% rooting percentage) and acclimatized ex vitro in floating perlite, with 90% acclimatization percentage. To assess the genetic fidelity between the mother plant and in vitro propagated plants, sequence-related amplified polymorphism (SRAP) markers were used. All banding profiles from the micropropagated plants were monomorphic and similar to those of the mother plant indicating 100% similarity. Regarding the polyphenolic profile, gallic, protocatechuic, p-hydroxybenzoic, vanillic, chlorogenic, caffeic, syringic, p-coumaric and ferulic acid were present in different amounts (2.3–2690.3 μg g⁻¹ dry plant), according to the extracted matrix. Aglicons and glycosides of different classes of flavonoids were also identified. The rhizome extracts (both from in vitro and field grown plants) contained resveratrol, a stilbene compound with high antioxidant properties, ranging between 229.4 to 371.7 μg g⁻¹ plant. Our results suggest that in vitro propagation of Rheum rhabarbarum L. represents a reliable alternative to obtain a large number of true-to-type planting material with high bioactive compound content of this valuable nutritional and medicinal species.

中文翻译：

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大黄的微繁殖，遗传保真度和酚类化合物的生产

在这项研究中，开发了一种有效的大黄鼠李微繁殖方案。中在乘法阶段获得体外大黄植物（增殖率：5.0±0.5）植根于体外（96％生根率）和水土不服试管外在浮动珍珠岩中，具有90％的驯化百分比。为了评估母体植物与体外繁殖植物之间的遗传保真度，使用了与序列相关的扩增多态性（SRAP）标记。来自微繁殖植物的所有条带图都是单态的，与母本植物的图谱相似，表明100％相似。关于多酚特性，没食子酸，没食子酸，对羟基苯甲酸，香草酸，绿原酸，咖啡酸，丁香酸，对香豆酸和阿魏酸的含量不同（2.3–2690.3μgg ^-1干燥的植物），根据提取的基质。还鉴定了不同种类的类黄酮的葡聚糖和糖苷。根茎提取物（来自体外和田间生长的植物）都含有白藜芦醇，一种具有高抗氧化特性的二苯乙烯化合物，在229.4至371.7μgg ^-1植物之间。我们的结果表明，大黄大黄的体外繁殖代表了一种可靠的替代方法，可以获得大量具有这种有价值的营养和药用物种高生物活性化合物含量的真型种植材料。