Male reproductive impairment is responsible for at least 50% of cases of couple infertility. Nuclear factor‐kappa B (NF‐κB) has been functionally linked to germ cell apoptosis, which may affect human fertility. The aim of this study was to determine the association between the rs28362491 SNP of the NF‐κB1 gene and infertility in Egyptian men. In this case–control study, semen and blood samples of 247 infertile men, constituting the case group, and of 113 fertile healthy men as the control group were analysed. All study participants were genotyped for polymorphism of the NF‐κB1 gene (rs28362491) by the polymerase chain reaction—restriction fragment length polymorphism (PCR‐RFLP) technique. Heterozygous I/D genotype of the NF‐κB1 rs28362491 polymorphism was associated with a significantly lower risk of poor semen quality, including asthenozoospermia, astheno–teratozoospermia, and oligo–astheno–teratozoospermia, when compared to I/I genotype (odds ratio = 0.25, 0.26, 0.18, p  < .0005, <.0005, <.0005) respectively. Overall, the presence of the D allele was associated with a significantly decreased risk of poor sperm quality as compared to the I allele (odds ratio = 0.56, 0.64, 0.49, p  = .050, .038, .001). In conclusion, these results suggest that heterozygosity of the NF‐κB1 gene may play a protecting role against male infertility in Egyptians.

中文翻译：

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NF-κB1 Rs28362491 多态性在埃及人口男性生育力中的可能作用。

男性生殖障碍是造成至少 50% 的夫妻不孕症的原因。核因子-κB (NF-κB) 在功能上与生殖细胞凋亡有关，这可能会影响人类的生育能力。本研究的目的是确定 NF-κB1 基因的 rs28362491 SNP 与埃及男性不育症之间的关联。在这项病例对照研究中，分析了构成病例组的 247 名不育男性和作为对照组的 113 名可育健康男性的精液和血液样本。通过聚合酶链反应-限制性片段长度多态性 (PCR-RFLP) 技术对所有研究参与者进行 NF-κB1 基因 (rs28362491) 多态性的基因分型。NF-κB1 rs28362491 多态性的杂合 I/D 基因型与精液质量差的风险显着降低有关，包括弱精子症，p  < .0005, <.0005, <.0005)。总体而言，与 I 等位基因相比，D 等位基因的存在与精子质量差的风险显着降低相关（优势比 = 0.56、0.64、0.49、p  = 0.050、0.038、0.001）。总之，这些结果表明 NF-κB1 基因的杂合性可能对埃及人的男性不育症起保护作用。