We aimed to investigate the influence of titanium surface characteristics on epigenetic mechanisms and DNA damage/repair pathways. Osteoblast‐like cells (MG63) were incubated on glass, smooth titanium, and minimally rough titanium discs, respectively, for 0, 1, 6, and 24 hr. The presence of double‐stranded DNA damage (γH2AX), DNA repair (Chk2), and epigenetic markers (AcH3 & DNMT1) were investigated using immunofluorescence. There were no Chk2‐positive cells on the minimally rough titanium surfaces at all‐time points, in comparison to glass and smooth titanium. Total γH2AX‐positive cells on minimally rough titanium gradually decreased as incubation time increased, on the contrary to smooth titanium. Minimally rough titanium surfaces induced cytoplasmic staining of DNMT1 up to 99% at 24 hr. For epigenetic markers related to the DNA damage/repair pathway, minimally rough titanium surfaces showed the lower percentage of AcH3‐positive cells compared to glass and smooth titanium surface. The findings in the current study show that titanium surface characteristics indeed influence DNA damage and the DNA repair pathway, including epigenetic factors.

中文翻译：

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成骨细胞表观遗传变化响应钛表面特征。

我们旨在研究钛表面特征对表观遗传机制和 DNA 损伤/修复途径的影响。成骨细胞样细胞 (MG63) 分别在玻璃、光滑钛和最小粗糙钛盘上孵育 0、1、6 和 24 小时。使用免疫荧光研究双链 DNA 损伤 (γH2AX)、DNA 修复 (Chk2) 和表观遗传标记 (AcH3 和 DNMT1) 的存在。与玻璃和光滑的钛相比，在所有时间点上，在最不粗糙的钛表面上都没有 Chk2 阳性细胞。与光滑钛相反，随着孵育时间的增加，最小粗糙钛上的总 γH2AX 阳性细胞逐渐减少。最小粗糙的钛表面在 24 小时时诱导 DNMT1 的细胞质染色高达 99%。对于与 DNA 损伤/修复途径相关的表观遗传标记，与玻璃和光滑钛表面相比，极不粗糙的钛表面显示出较低的 AcH3 阳性细胞百分比。当前研究的结果表明，钛表面特征确实会影响 DNA 损伤和 DNA 修复途径，包括表观遗传因素。