Abstract Dracocephalum kotschyi Boiss. and D. polychaetum Bornm. (Lamiaceae family) are wild medicinal herbs native to Iran and a source of potential bioactive compounds for pharmaceutical and food industries. For their bulk production, the present work aimed to investigate the effects of explants, different plant growth regulators (PGRs), and photoperiod conditions on the callus induction and cell suspension of two Dracocephalum species. A factorial experiment method based on a completely randomized design with three replications was chosen. The best conditions for callus growth obtained on the 45th day from hypocotyl, stem, and leaf explants in Murashige and Skoog (MS) medium supplemented by 1.0 mg. L−1 1-naphthalene acetic acid (NAA) and 4.5 mg. L−1 6-benzylaminopurine (BAP) in both D. polychaetum and D. kotschyi, while the best condition for callus growth from root explant was MS medium supplemented by 1.0 mg. L−1 NAA and 6.0 mg. L−1 BAP in both species. The cell suspension cultures of two species showed the highest growth rate in B5 media supplemented by 1.0 mg L−1 NAA and 2.5 mg L−1 BAP, with friable callus. The methanolic extracts of D. kotschyi and D. polychaetum cells were then determined by high performance liquid chromatography and recognized as a valuable source of such phenolics as rosmarinic acid, naringin, epicatechin, thymol, carvacrol, rutin, p-coumaric acid, gallic acid, benzoic acid, cinnamic acid, apigenin, quercetin, chlorogenic acid, and 4-hydroxybenzoic acid.

中文翻译：

---

Dracocephalum polychaetum Bornm 的Callogenesis 优化和细胞悬浮培养建立。和 Dracocephalum kotschyi Boiss。：次生代谢物生产的体外方法

摘要 Dracocephalum kotschyi Boiss。和 D. polychaetum Bornm。（唇形科）是原产于伊朗的野生药材，是制药和食品工业潜在生物活性化合物的来源。对于它们的批量生产，目前的工作旨在研究外植体、不同的植物生长调节剂 (PGR) 和光周期条件对两种 Dracocephalum 物种的愈伤组织诱导和细胞悬浮的影响。选择了基于具有三个重复的完全随机设计的析因实验方法。在第 45 天从下胚轴、茎和叶外植体在 Murashige 和 Skoog (MS) 培养基中获得的愈伤组织生长的最佳条件，辅以 1.0 毫克。L-1 1-萘乙酸 (NAA) 和 4.5 mg。D. polychaetum 和 D. kotschyi 中的 L−1 6-苄氨基嘌呤 (BAP)，而根外植体愈伤组织生长的最佳条件是添加 1.0 mg 的 MS 培养基。L-1 NAA 和 6.0 毫克。两个物种中的 L-1 BAP。两个物种的细胞悬浮培养物在补充有 1.0 mg L-1 NAA 和 2.5 mg L-1 BAP 的 B5 培养基中显示出最高的生长速度，并且具有易碎的愈伤组织。D. kotschyi 和 D. polychaetum 细胞的甲醇提取物随后通过高效液相色谱法测定，并被认为是迷迭香酸、柚皮苷、表儿茶素、百里酚、香芹酚、芦丁、对香豆酸、没食子酸等酚类物质的宝贵来源、苯甲酸、肉桂酸、芹菜素、槲皮素、绿原酸和4-羟基苯甲酸。两个物种的细胞悬浮培养物在补充有 1.0 mg L-1 NAA 和 2.5 mg L-1 BAP 的 B5 培养基中显示出最高的生长速度，并且具有易碎的愈伤组织。D. kotschyi 和 D. polychaetum 细胞的甲醇提取物随后通过高效液相色谱法测定，并被认为是迷迭香酸、柚皮苷、表儿茶素、百里酚、香芹酚、芦丁、对香豆酸、没食子酸等酚类物质的宝贵来源、苯甲酸、肉桂酸、芹菜素、槲皮素、绿原酸和4-羟基苯甲酸。两个物种的细胞悬浮培养物在补充有 1.0 mg L-1 NAA 和 2.5 mg L-1 BAP 的 B5 培养基中显示出最高的生长速度，并且具有易碎的愈伤组织。D. kotschyi 和 D. polychaetum 细胞的甲醇提取物随后通过高效液相色谱法测定，并被认为是迷迭香酸、柚皮苷、表儿茶素、百里酚、香芹酚、芦丁、对香豆酸、没食子酸等酚类物质的宝贵来源、苯甲酸、肉桂酸、芹菜素、槲皮素、绿原酸和4-羟基苯甲酸。