Abstract Contamination of Penicillium expansum has dramatic impacts on the development of the fruit and vegetable industry. In this study, the antifungal effect and its mechanism of curcumin mediated photodynamic technology (PDT) against P. expansum spores was investigated. Results indicated that PDT inactivated 99.7 % spores and inhibited spore germination, mycelial growth and disease severity of the blue mold rot on apple fruit. PDT induced the generation of reactive oxygen species (ROS), which caused oxidative damage of spores and further disrupted cellular structure. In addition, excessive ROS triggered the antioxidant defense systems of spores. Moreover, ultimate result of cell death induced by PDT was apoptosis for short time illumination, and necrosis for long time. These results can be an asset to exploit PDT for further application in controlling harvest diseases of the fruit and vegetable.

中文翻译：

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姜黄素介导的光动力技术对膨胀青霉的抗真菌作用及可能机制

摘要 膨胀青霉的污染对果蔬产业的发展产生了巨大的影响。在这项研究中，研究了姜黄素介导的光动力技术（PDT）对P. expansum孢子的抗真菌作用及其机制。结果表明，PDT 灭活了 99.7% 的孢子，并抑制了苹果果实上蓝霉腐病的孢子萌发、菌丝生长和病害严重程度。PDT 诱导活性氧 (ROS) 的产生，导致孢子氧化损伤并进一步破坏细胞结构。此外，过量的 ROS 会触发孢子的抗氧化防御系统。此外，PDT诱导细胞死亡的最终结果是短时间照射凋亡，长时间照射坏死。