In order to characterize the mechanisms controlling plasticity in the mouse visual cortex, we used, for the first time on brain samples, an unconventional proteomic approach to separate acid-extracted proteins by bi-dimensional electrophoresis (AUT/SDS or AUT/AU gels). The analysis was performed on high plasticity critical period young vs. low plasticity adult, and on fluoxetine-induced high plasticity vs. low plasticity untreated adult mice. Mass spectrometry allowed for the identification of 11 proteins that are differentially expressed between critical period and adult mice, and 5 between fluoxetine-treated and control adult mice. We then focused on cofilin 1, as the intensity level of the corresponding spot on 2D gels was higher in both high plasticity conditions. Western blot showed that cofilin 1 expression is dynamically regulated during postnatal life, reaching a peak at the critical period, and decreasing at adult stage, and that it increases in fluoxetine-treated vs. untreated adult mice. In summary, by using a 2D gel electrophoresis differential approach on basic proteins followed by mass spectrometry and immunoblot analysis, we identified cofilin 1 as a potential candidate controlling plasticity levels of the mouse visual cortex.

中文翻译：

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鉴定cofilin 1为与小鼠视觉皮层可塑性相关的候选蛋白。

为了表征控制小鼠视觉皮层可塑性的机制，我们首次在脑样品上使用了一种非常规的蛋白质组学方法，通过二维电泳（AUT / SDS或AUT / AU凝胶）分离酸提取的蛋白质。 。该分析是针对高可塑性关键时期的年轻小鼠与低可塑性成年小鼠，以及氟西汀诱导的高可塑性与低可塑性成年小鼠进行的。质谱法可以鉴定在关键时期和成年小鼠之间差异表达的11种蛋白质，以及在氟西汀治疗和对照成年小鼠之间差异表达的11种蛋白质。然后，我们着眼于cofilin 1，因为在两种高可塑性条件下，二维凝胶上相应斑点的强度都较高。蛋白质印迹表明，cofilin 1的表达在出生后的生命中受到动态调节，在关键时期达到峰值，在成年期下降，并且在经过氟西汀治疗的成年小鼠中，其表达增加。总而言之，通过对基本蛋白质使用二维凝胶电泳差分方法，然后进行质谱分析和免疫印迹分析，我们确定cofilin 1是控制小鼠视觉皮层可塑性水平的潜在候选者。