Trayodashang guggulu is an important Ayurvedic polyherbal formulation containing babula (stem bark of Acacia nilotica), ashwagandha (roots of Withaniasomnifera), hapusa (fruits of Juniperuscommunis), guduchi (stem of Tinosporacordifolia), shatavari (roots of Aspargusrecemosus), gokshuara (fruits of Tribulusterrestris), vradadaru (roots of Argyeria nervosa), rasana (roots and leaves of Pluchealanceolata), satavha (fruits of Anethumsowa), sati (rhizome of Hidichiumspicatum), yavani (Trachyspermumammi fruits), sunthi (rhizome of Zingiberofficinale), shuddhaofficinale guggulu (exudates of Commipheramukul) and goghrat (ghee). Trayodashang guggulu is used in the Ayurvedic system of medicine for the treatment of various inflammatory conditions such as arthritis and associated pain. Hence, the present study investigated the in-vitro anti-inflammatory and antioxidant activities of trayodashang guggulu. Trayodashang guggulu was standardized as per standard procedures and TLC profile was carried as per Ayurvedic Pharmacopoeia of India. In-vitro anti-inflammatory activity of its aqueous extract (AqTG) (in different concentrations) was evaluated by assaying inhibition of albumin denaturation, membrane stabilization (hypotonicity-induced haemolysis), anti-lipoxygenase and antiproteinase activities. The in-vitro antioxidant effect was evaluated by various in-vitro methods viz. DPPH (1, 1-diphenyl-2-picryl-hydrazyl) and hydroxyl radical scavenging and reducing power assay and total phenolic and flavonoids contents. Trayodashang guggulu was confirmed as per pharmacopoeial standards. It showed marked scavenging effect on DPPH and hydroxyl radicals and exhibited strong reducing potential. It also showed inhibition of membrane stabilizing, protein denaturation inhibitory, anti-lipoxygenase and anti-proteinase activities. Trayodashang guggulu exhibited in-vitro anti-inflammatory and antioxidant activities. This preliminary study supports the therapeutic claim of the formulation as an anti-inflammatory drug in the Ayurvedic system of medicine and advocates its use in inflammatory conditions. In view of the in-vitro results, further in-vivo investigations are needed to confirm and strengthen the anti-inflammatory activity of trayodashang guggulu in pain, inflammation and arthritis.

Trayodashang guggulu是含有babula一个重要的阿育吠陀多草药制剂（干树皮金合欢），南非醉茄（根Withaniasomnifera），hapusa（果实Juniperuscommunis），guduchi（茎的Tinosporacordifolia），芦笋草（根Aspargusrecemosus），gokshuara（果实Tribulusterrestris），vradadaru（根Argyeria性厌食症），rasana（根和叶Pluchealanceolata），satavha（水果Anethumsowa），殉夫（根茎Hidichiumspicatum），yavani（Trachyspermumammi水果），sunthi（根茎Zingiberofficinale），shuddhaofficinale guggulu（的渗出物Commipheramukul）和goghrat（酥油）。Trayodashang guggulu用于印度草药医学系统中，用于治疗各种炎性疾病，例如关节炎和相关的疼痛。因此，本研究调查了延胡索蛇毒的体外抗炎和抗氧化活性。Trayodashang guggulu按照标准程序进行了标准化，并且TLC谱按照印度阿育吠陀药典进行。体外通过测定白蛋白变性，膜稳定（低渗诱导的溶血），抗脂氧合酶和抗蛋白酶活性的抑制作用来评估其水提取物（AqTG）（不同浓度）的抗炎活性。的体外抗氧化作用是通过各种评价体外方法即 DPPH（1，1-二苯基-2-picryl-肼基）和羟基自由基清除和还原能力测定以及总酚和类黄酮含量。Trayodashang古古鲁已根据药典标准确认。它对DPPH和羟基具有明显的清除作用，并具有很强的还原能力。它还显示出对膜稳定，蛋白变性抑制，抗脂氧合酶和抗蛋白酶活性的抑制作用。Trayodashang guggulu表现出体外抗炎和抗氧化活性。该初步研究支持该制剂在阿育吠陀医学系统中作为抗炎药的治疗主张，并提倡在炎症性疾病中使用该制剂。鉴于体外结果，需要进一步的体内研究以确认和增强雷竹大骨胶的抗炎活性。 疼痛，发炎和关节炎。