Resistant starch (RS) also known as healthy starch has shown several health benefits. Enhancing the RS through genetic modification approaches has huge commercial importance. Regulatory RNA like long non-coding RNA (lncRNA) plays an important role in gene regulation. In this study, we mined 63 transcriptome datasets of wheat belonging to 35 genotypes representing two seed developmental stages. Contrasting expression of a subset of. lncRNAs in RS mutant lines compared to parent wheat variety 'C 306' signifies their probable role in RS biosynthesis. Further, lncRNA- TCONS_00130663 showed strong positive correlation (r2 = 1) with LYPL gene and strong negative correlation with SBEIIb (r2 = -0.94). We found TCONS_00130663 as positive regulator of LYPL gene through interaction with miR1128. Based on relative expression, in-silico interaction and DSC analysis we hypothesize the dual role of TCONS_00130663 in RS type 2 and type 5. The study provides a useful resource for functional mechanism of lncRNAs.

中文翻译：

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调控面包小麦 (Triticum aestivum L.) 抗性淀粉生物合成的长链非编码 RNA 的鉴定和表征。

抗性淀粉 (RS) 也称为健康淀粉，已显示出多种健康益处。通过基因改造方法增强 RS 具有巨大的商业重要性。长链非编码 RNA (lncRNA) 等调控 RNA 在基因调控中发挥着重要作用。在这项研究中，我们挖掘了 63 个小麦转录组数据集，它们属于代表两个种子发育阶段的 35 个基因型。子集的对比表达。与亲本小麦品种“C 306”相比，RS 突变系中的 lncRNA 表明它们可能在 RS 生物合成中发挥作用。此外，lncRNA-TCONS_00130663 与 LYPL 基因呈强正相关（r2 = 1），与 SBEIIb 呈强负相关（r2 = -0.94）。我们通过与 miR1128 的相互作用发现 TCONS_00130663 作为 LYPL 基因的正调节因子。基于相对表达，