The effect of estrogens on Oryzias curvinotus juveniles were investigated by sequencing the transcriptome of O. curvinotus juveniles exposed to 17 β - estradiol for 24 h. A total of 69,071,524 and 71,210,528 raw reads were obtained for the control group (NC) and 17 β - estradiol exposure group (E2), respectively. After de novo assembly, total 133,210 unigenes were identified, and 85,837 unigenes (64.44% of 133,210) were annotated. Analysis of the transcriptome showed that exposure to 2 μg/L 17 β - estradiol led to the up-regulation of 19 genes and down-regulation of 18 genes. The eef1b and rps4x was most suitable as controls for quantitative real-time PCR (qPCR) using Reffinder. Different expression genes enrichment analysis found that exposed to 2 μg/L 17 β - estradiol affected various physiological processes, including spliceosome, phototransduction, amino sugar and nuclear sugar metabolism, hypotaurine metabolism, and renin-angiotensin system, etc. Exposing O. curvinotus juveniles to increasing concentrations of 17 β - estradiol (2 ng/L, 20 ng/L, 200 ng/L and 2 μg/L) led to significant up-regulation of vitellogenins (vtgs) and choriogenins (chgs) mRNA expression. The present study is the first high-throughput transcriptome sequencing of O. curvinotus juveniles, which will be useful for future functional analysis of genes related to environmental estrogen exposed, and development of biomarkers.

通过对暴露于17β-雌二醇24小时的弯曲。的转录组进行测序，研究了雌激素对弯曲ju的影响。对照组（NC）和17β-雌二醇暴露组（E2）分别获得了69,071,524和71,210,528原始读数。从头开始组装后，总共鉴定了133,210个单基因，并注释了85,837个单基因（占133,210个的64.44％）。转录组分析表明，暴露于2μg/ L 17β-雌二醇可导致19个基因的上调和18个基因的下调。该eef1b和RPS4X最适合用作使用Reffinder进行定量实时PCR（qPCR）的对照。不同的表达基因富集分析发现，暴露至2μg/ L 17β -雌二醇影响各种生理过程，包括剪接，光转导，氨基糖和核糖代谢，亚牛磺酸代谢和肾素-血管紧张素系统，等等暴露O. curvinotus幼鱼至17β的增加浓度-导致的显著上调雌二醇（2纳克/升，20纳克/升，200纳克/升和2μg/ L）vitellogenins（VTGS）和choriogenins（CHGS）mRNA的表达。本研究是曲霉O. vivinotus的第一个高通量转录组测序 幼虫，这对于与环境雌激素暴露有关的基因的未来功能分析以及生物标记物的开发将是有用的。